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- Table of Contents
HLA-DQB1 · Western blot design guide
HLA-DQB1 Western Blot Planning Guide
Plan a HLA-DQB1 Western blot around the catalog-observed 30 kDa band, image-backed A00106-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
HLA-DQB1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 30 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Lung | |
| Negative control | Oral mucosa |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 30 kDa |
| ⓘ | Localization | Cell membrane / Endoplasmic reticulum membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated HLA-DQB1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00106-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected HLA-DQB1 Western Blot Band Size?
Use the product-observed 30 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 30 kDa | Matches the authoritative product WB observation. |
| 30 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected HLA-DQB1 appearancePlan around 30 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 30 kDa; use this as the primary experimental expectation. |
| Calculated mass | 30 kDa from UniProt P01920; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00106-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 30 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing HLA-DQB1 band
A00106-1 · HLA-DQB1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HLA-DQB1 Western blot
🧪Use Lung as the first positive-control candidate and Oral mucosa as the HPA Not detected negative candidate.
Positive control: Lung (High)
Negative control: Oral mucosa (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for HLA-DQB1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced HLA-DQB1 Western Blot Tips
Deeper troubleshooting and optimisation questions for HLA-DQB1, answered from its protein features.
Which band should guide the blot?
Use 30 kDa, the observation attached to the authoritative A00106-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 30 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Oral mucosa as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for HLA-DQB1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00106-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
HLA-DQB1 Western Blot Reagents
Human-reactive HLA-DQB1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-HLA-DQB1 Antibody Picoband®
Cat # A00106-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P01920
- Human Protein Atlas — HLA-DQB1
- A00106-1 product record
- PMC6823813 — Proteomic studies of putative molecular signatures for biological effects by Korean Red Ginseng (Journal of ginseng research, 2019)