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- Table of Contents
HSF2 · Western blot design guide
HSF2 Western Blot Planning Guide
Plan a HSF2 Western blot around the catalog-observed 60.3 kDa band, image-backed A04692 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
HSF2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 60.3 kDa observed | |
| Observed band | 60.3 kDa observed | |
| Gel | 12-15% | |
| Positive control | Testis | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 60.3 kDa |
| ⓘ | Localization | Cytoplasm / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated HSF2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Testis |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A04692 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected HSF2 Western Blot Band Size?
Use the product-observed 60.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 60.3 kDa | Matches the authoritative product WB observation. |
| 60.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected HSF2 appearancePlan around 60.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 60.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 60.3 kDa from UniProt Q03933; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A04692. |
| Situation | Likely cause | Next action |
|---|---|---|
| 60.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing HSF2 band
A04692 · HSF2Observed 60.3 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HSF2 Western blot
🧪Use Testis as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Testis (High)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for HSF2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | Low | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
| Adipose tissue | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced HSF2 Western Blot Tips
Deeper troubleshooting and optimisation questions for HSF2, answered from its protein features.
Which band should guide the blot?
Use 60.3 kDa, the observation attached to the authoritative A04692 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 60.3 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for HSF2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A04692 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
HSF2 Western Blot Reagents
Human/Mouse/Rat-reactive HSF2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-HSF2 Antibody Picoband®
Cat # A04692
Real WB data
Anti-Heat shock factor protein 2 HSF2 Antibody Picoband®
Cat # PA1607
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.