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- Table of Contents
ING1 · Western blot design guide
ING1 Western Blot Planning Guide
Plan an ING1 Western blot around the catalog-observed 46.7 kDa band, image-backed PB9644 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ING1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 46.7 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Lung | |
| Negative control | Target knockdown/knockout |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 46.7 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated ING1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bone marrow |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9644 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ING1 Western Blot Band Size?
Use the product-observed 46.7 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 46.7 kDa | Matches the authoritative product WB observation. |
| 46.7 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ING1 appearancePlan around 46.7 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 46.7 kDa; use this as the primary experimental expectation. |
| Calculated mass | 46.7 kDa from UniProt Q9UK53; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9644. |
| Situation | Likely cause | Next action |
|---|---|---|
| 46.7 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ING1 band
PB9644 · ING1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ING1 Western blot
🧪Use Lung as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.
Positive control: Lung (High)
Negative control: Target knockdown/knockout
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ING1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|
Section 3
Advanced ING1 Western Blot Tips
Deeper troubleshooting and optimisation questions for ING1, answered from its protein features.
Which band should guide the blot?
Use 46.7 kDa, the observation attached to the authoritative PB9644 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 46.7 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use a target knockdown/knockout control when no HPA Not detected tissue is available.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ING1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9644 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ING1 Western Blot Reagents
Human-reactive ING1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-ING1 Antibody Picoband®
Cat # PB9644
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UK53
- Human Protein Atlas — ING1
- PB9644 product record
- PMC4239040 — MiR-371-5p facilitates pancreatic cancer cell proliferation and decreases patient survival (PloS one, 2014)
- PMC10111810 — Analysis of patients with colorectal cancer shows a specific increase in serum anti-ING1 autoantibody levels (BMC cancer, 2023)
- PMC11847347 — Cancer-secreted exosomal miR-1825 induces angiogenesis to promote colorectal cancer metastasis (Cancer cell international, 2025)
