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- Table of Contents
ITPR3 · Western blot design guide
ITPR3 Western Blot Planning Guide
Plan an ITPR3 Western blot around the catalog-observed 304.1 kDa band, image-backed PB9428 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ITPR3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 304.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Small intestine | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 304.1 kDa |
| ⓘ | Localization | Endoplasmic reticulum membrane / Cytoplasmic vesicle, secretory vesicle membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated ITPR3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebellum |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9428 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ITPR3 Western Blot Band Size?
Use the product-observed 304.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 304.1 kDa | Matches the authoritative product WB observation. |
| 304.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ITPR3 appearancePlan around 304.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 304.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 304.1 kDa from UniProt Q14573; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9428. |
| Situation | Likely cause | Next action |
|---|---|---|
| 304.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ITPR3 band
PB9428 · ITPR3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ITPR3 Western blot
🧪Use Small intestine as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Small intestine (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ITPR3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Small intestine | Reported tissue cells | High | Protein (HPA) | HPA → |
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced ITPR3 Western Blot Tips
Deeper troubleshooting and optimisation questions for ITPR3, answered from its protein features.
Which band should guide the blot?
Use 304.1 kDa, the observation attached to the authoritative PB9428 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 304.1 kDa expectation.
Which positive control should I start with?
Start with Small intestine, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for ITPR3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9428 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ITPR3 Western Blot Reagents
Human/Mouse/Rat-reactive ITPR3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-ITPR3 Antibody Picoband®
Cat # PB9428
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q14573
- Human Protein Atlas — ITPR3
- PB9428 product record
- PMC13254598 — Regulation of chromatin dynamics by a calcium-dependent nucleoskeleton (The Journal of biological chemistry, 2026)
- PMC7906004 — Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis (Gut, 2021)
- PMC7877014 — ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma (Journal of experimental & clinical cancer research : CR, 2021)