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Product Info Summary
| SKU: | PB9428 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ITPR3 Antibody Picoband®
SKU/Catalog Number
PB9428
PB0409 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ITPR3 Antibody Picoband® catalog # PB9428. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ITPR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9428)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9428 is reactive to ITPR3 in Human, Mouse, Rat
Observed Molecular Weight
270-300 kDa
Calculated molecular weight
304.1 kDa
Background of ITPR3
ITPR3 encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. ITP3 channels serve an important role in the taste transduction pathway of sweet, bitter and umami tastes the gustatory system. ITP3 channels allow the flow of Calcium out of the endoplasmic reticulum in response to IP3. Calcium cations result in the activation of TRPM5 which leads to a depolarisation generating potential and an action potential.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9428 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell
IHC: mouse intestine tissue, rat intestine tissue, human lung cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ITPR3 using anti-ITPR3 antibody (PB9428).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPR3 antigen affinity purified polyclonal antibody (Catalog # PB9428) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPR3 at approximately 270-300 kDa. The expected band size for ITPR3 is at 304 kDa.
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IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428).
ITPR3 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428).
ITPR3 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428).
ITPR3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-ITPR3 Antibody Picoband® (PB9428)
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Customer Q&As
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7 Customer Q&As for Anti-ITPR3 Antibody Picoband®
Question
Could you advise on the name, state, and concentration of preservatives in PB9428?
Verified customer
Asked: 2020-03-31
Answer
The Anti-ITPR3 Antibody Picoband PB9428 contains 0.02% sodium azide and it was powder resolved in antibody solution before lyophilizing.
Boster Scientific Support
Answered: 2020-03-31
Question
Will anti-ITPR3 antibody PB9428 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to the expression profile of cervix carcinoma erythroleukemia, ITPR3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-ITPR3 antibody PB9428 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-ITPR3 antibody PB9428 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The anti-ITPR3 antibody (PB9428) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-08
Question
I am interested in to test anti-ITPR3 antibody PB9428 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ITPR3 antibody PB9428 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Bhatt
Verified customer
Asked: 2019-05-22
Answer
The products we sell, including anti-ITPR3 antibody PB9428, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-22
Question
Does anti-ITPR3 antibody PB9428 work on monkey WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
Our lab technicians have not validated anti-ITPR3 antibody PB9428 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-ITPR3 antibody PB9428 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-17
Question
A customer here would like to know what kind of rat tissue was tested for WB application of this PB9428 Anti-ITPR3 Picoband
Verified Customer
Verified customer
Asked: 2014-09-05
Answer
None of the rat samples we tested successfully for WB using PB9428. We listed rat as tested species because it worked well on rat samples for IHC application. You can find the detailed information at "Assay Dilutions Overview" section. "Immunohistochemistry(Paraffin-embedded Section), 0.5-1?g/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5?g/ml, Human"
Boster Scientific Support
Answered: 2014-09-05
Question
I see that the anti-ITPR3 antibody PB9428 works with WB, what is the protocol used to produce the result images on the product page?
P. Yang
Verified customer
Asked: 2013-08-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-08-12
