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- Table of Contents
INSIG1 Antibodies
AND INSIG1 ELISA kits, INSIG1 Proteins
Many biological assays use antibodies to detect INSIG1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with INSIG1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop INSIG1 antibodies...
Designing a Western blot for INSIG1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the INSIG1 Western Blot GuideINSIG1 Infographic by Boster Bio
All INSIG1 Products
Product
Figures
Specificity
Applications
Price
Human
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A02026-2
100 µg/vial
Cat # A02026-2
100 µg/vial
INSIG1 Overview
Facts about Insulin-induced gene 1 protein.
Insulin-induced gene 1 protein (INSIG1)
Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs).
Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78.
Gene Information
| Human | |
|---|---|
| Gene Name: | INSIG1 |
| Uniprot: | O15503 |
| Entrez: | 3638 |
Family
| Belongs to: |
|---|
| INSIG family |
Alternative Names
CL6; CL-6; INSIG-1 membrane protein; INSIG-1; insulin induced gene 1; insulin-induced gene 1 protein; MGC1405
Molecular Weight
Mass (kDA):
29.987 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 7q36.3 |
| Sequence: | 7; NC_000007.14 (155297772..155310235) |
Tissue Specificity
Expressed in all tissues tested with highest expression in the liver.
Subcellular Localizations
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Diseases
- Obesity
- Diabetes Mellitus
- Malignant Neoplasms
- Liver Carcinoma
- Diabetes Mellitus, Non-insulin-dependent
- Insulin Resistance
- Dyslipidemias
- Neoplasms
- Hypercholesterolemia
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Fatty Liver
- Cleft Palate
Interacting Proteins
- AMFR
Pathways
- Cholesterol Homeostasis
- Lipid Homeostasis
- Lactation
- Transport
- Rna Interference
- Proteolysis
- Cell Cycle
- Cell Growth
- Glucose Homeostasis
- Cell Proliferation
- Parturition
- Pathogenesis
PTMs
- Ubiquitination
- Phosphorylation
- Cleavage
- Reduction
Research Areas
- Cholesterol Metabolism
- ER Markers
- Lipid and Metabolism
- Signal Transduction
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/INSIG1
bosterbio.com/bosterbio-gene-info-cards/INSIG1
References
PMID: 9268630 by Peng Y., et al. Cloning, human chromosomal assignment, and adipose and hepatic expression of the CL-6/INSIG1 gene.
PMID: 12202038 by Yang T., et al. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.