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- Table of Contents
KCNA3 · Western blot design guide
KCNA3 Western Blot Planning Guide
Plan a KCNA3 Western blot around the catalog-observed 63.8 kDa band, image-backed PB9650 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
KCNA3 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 63.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cerebellum | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 63.8 kDa |
| ⓘ | Localization | Cell membrane / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for KCNA3 Western blot
🧪Use Cerebellum as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Cerebellum (Medium)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for KCNA3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Hippocampus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 1
What Is the Expected KCNA3 Western Blot Band Size?
Use the product-observed 63.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 63.8 kDa | Matches the authoritative product WB observation. |
| 63.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected KCNA3 appearancePlan around 63.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 63.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 63.8 kDa from UniProt P22001; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9650. |
| Situation | Likely cause | Next action |
|---|---|---|
| 63.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing KCNA3 band
PB9650 · KCNA3Observed 63.8 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated KCNA3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Caudate |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9650 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced KCNA3 Western Blot Tips
Deeper troubleshooting and optimisation questions for KCNA3, answered from its protein features.
Which band should guide the blot?
Use 63.8 kDa, the observation attached to the authoritative PB9650 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 63.8 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for KCNA3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9650 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC5204372, PMC11696611, PMC10681899.
Boster reagents
KCNA3 Western Blot Reagents
Human/Mouse/Rat-reactive KCNA3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-KCNA3 Antibody Picoband®
Cat # PB9650
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P22001
- Human Protein Atlas — KCNA3
- PB9650 product record
- PMC5204372 — Ionic immune suppression within the tumour microenvironment limits T cell effector function.
- PMC11696611 — E4F1 coordinates pyruvate metabolism and the activity of the elongator complex to ensure translation fidelity during brain development.
- PMC10681899 — E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation.