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- Table of Contents
MNT · Western blot design guide
MNT Western Blot Planning Guide
Plan a MNT Western blot around the catalog-observed 62.3 kDa band, image-backed A03357-3 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
MNT Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 62.3 kDa observed | |
| Observed band | 62.3 kDa observed | |
| Gel | 12-15% | |
| Positive control | No defensible HPA tissue; use knockout/knockdown | |
| Negative control | Use knockout/knockdown or orthogonal negative |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 62.3 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated MNT Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Known-positive human lysate |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03357-3 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected MNT Western Blot Band Size?
Use the product-observed 62.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 62.3 kDa | Matches the authoritative product WB observation. |
| 62.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected MNT appearancePlan around 62.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 62.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 62.3 kDa from UniProt Q99583; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03357-3. |
| Situation | Likely cause | Next action |
|---|---|---|
| 62.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing MNT band
A03357-3 · MNTObserved 62.3 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MNT Western blot
🧪No defensible HPA positive control was available; do not invent one.
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for MNT
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|
Undetected expression · recommended negative controls
The Human Protein Atlas exposes no structured IHC tissue profile for MNT, so tissue-level positive/negative controls cannot be listed here. Use a documented MNT-positive lysate (recombinant MNT or a known-expressing line) as the positive control and a MNT knockdown/knockout lysate as the negative control; confirm against the antibody datasheet.
| Tissue | Cell type | Level | Evidence | Source |
|---|
Section 3
Advanced MNT Western Blot Tips
Deeper troubleshooting and optimisation questions for MNT, answered from its protein features.
Which band should guide the blot?
Use 62.3 kDa, the observation attached to the authoritative A03357-3 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 62.3 kDa expectation.
Which positive control should I start with?
No defensible positive tissue was supplied; do not invent one.
Which negative control is defensible?
Use a target knockdown/knockout control when no HPA Not detected tissue is available.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for MNT Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03357-3 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
MNT Western Blot Reagents
Human-reactive MNT Western blot reagents with authoritative product imagery.
Real WB data
Anti-MNT Antibody Picoband®
Cat # A03357-3
Real WB data
Anti-MNT Antibody Picoband®
Cat # A03357-4
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.