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- Table of Contents
MTHFD1L · Western blot design guide
MTHFD1L Western Blot Planning Guide
Plan a MTHFD1L Western blot around the catalog-observed 105.8 kDa band, image-backed A06289-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
MTHFD1L Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 105.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Kidney | |
| Negative control | Target knockdown/knockout |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 105.8 kDa |
| ⓘ | Localization | Mitochondrion |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated MTHFD1L Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A06289-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected MTHFD1L Western Blot Band Size?
Use the product-observed 105.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 105.8 kDa | Matches the authoritative product WB observation. |
| 105.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected MTHFD1L appearancePlan around 105.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 105.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 105.8 kDa from UniProt Q6UB35; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A06289-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 105.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing MTHFD1L band
A06289-1 · MTHFD1L
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MTHFD1L Western blot
🧪Use Kidney as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.
Positive control: Kidney (High)
Negative control: Target knockdown/knockout
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for MTHFD1L
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|
Section 3
Advanced MTHFD1L Western Blot Tips
Deeper troubleshooting and optimisation questions for MTHFD1L, answered from its protein features.
Which band should guide the blot?
Use 105.8 kDa, the observation attached to the authoritative A06289-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 105.8 kDa expectation.
Which positive control should I start with?
Start with Kidney, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use a target knockdown/knockout control when no HPA Not detected tissue is available.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for MTHFD1L Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A06289-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
MTHFD1L Western Blot Reagents
Human/Mouse/Rat-reactive MTHFD1L Western blot reagents with authoritative product imagery.
Real WB data
Anti-MTHFD1L Antibody Picoband®
Cat # A06289-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q6UB35
- Human Protein Atlas — MTHFD1L
- A06289-1 product record
- PMC6700437 — Expression and Role of Methylenetetrahydrofolate Dehydrogenase 1 Like (MTHFD1L) in Bladder Cancer (Translational oncology, 2019)
- PMC9142831 — Activated brown adipose tissue releases exosomes containing mitochondrial methylene tetrahydrofolate dehydrogenase (NADP dependent) 1-like protein (MTHFD1L) (Bioscience reports, 2022)
- PMC6704443 — MTHFD1L, A Folate Cycle Enzyme, Is Involved in Progression of Colorectal Cancer (Translational oncology, 2019)