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- Table of Contents
MYH10 · Western blot design guide
MYH10 Western Blot Planning Guide
Plan a MYH10 Western blot around the catalog-observed 229 kDa band, image-backed A02681-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
MYH10 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 229 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Fallopian tube | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 229 kDa |
| ⓘ | Localization | Cell projection, lamellipodium / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated MYH10 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebellum |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02681-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected MYH10 Western Blot Band Size?
Use the product-observed 229 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 229 kDa | Matches the authoritative product WB observation. |
| 229 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected MYH10 appearancePlan around 229 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 229 kDa; use this as the primary experimental expectation. |
| Calculated mass | 229 kDa from UniProt P35580; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02681-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 229 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing MYH10 band
A02681-1 · MYH10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MYH10 Western blot
🧪Use Fallopian tube as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Fallopian tube (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for MYH10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced MYH10 Western Blot Tips
Deeper troubleshooting and optimisation questions for MYH10, answered from its protein features.
Which band should guide the blot?
Use 229 kDa, the observation attached to the authoritative A02681-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 229 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for MYH10 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02681-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
MYH10 Western Blot Reagents
Human/Mouse/Rat-reactive MYH10 Western blot reagents with authoritative product imagery.
Real WB data
Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband®
Cat # A02681-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P35580
- Human Protein Atlas — MYH10
- A02681-1 product record
- PMC13264122 — RNF126 mediates fetal growth restriction via ubiquitination-dependent degradation of the MYH9/MYH10 complex (iScience, 2026)
- PMC11147509 — Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation (eLife, 2024)
- PMC8875441 — MYH10 Governs Adipocyte Function and Adipogenesis through Its Interaction with GLUT4 (International journal of molecular sciences, 2022)
