NAE1 Antibodies

AND NAE1 ELISA kits, NAE1 Proteins

Many biological assays use antibodies to detect NAE1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NAE1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NAE1 antibodies...

We validate the specificity of these antibodies to NAE1 by testing them on tissues known to express NAE1 positively and negatively. Browse below to find the NAE1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for NAE1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the NAE1 Western Blot Guide

NAE1 Infographic by Boster Bio

All NAE1 Products

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Product

Figures

Specificity

Applications

Price

Anti-APPBP1/NAE1 Antibody Picoband®

Western blot analysis of APPBP1/NAE1 using anti-APPBP1/NAE1 antibody (PB9339)

Human, Mouse, Rat

$370
Cat # PB9339
100 μg/vial

APPBP1 (NAE1) (NM_003905) Human Over-expression Lysate

APPBP1 (NAE1) Human Over-expression Lysates NM_001018160

Human

$628
Cat # LS008883
20 µg, 100 µg

APPBP1 (NAE1) (NM_001018160) Human Recombinant Protein

Coomassie blue staining of purified NAE1 protein

$1249
Cat # PROTQ13564
20 µg

NAE1 Overview

Facts about NEDD8-activating enzyme E1 regulatory subunit.

NAE1 3D structure. Source: alphafold

NEDD8-activating enzyme E1 regulatory subunit (NAE1)

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP.

E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint.

Gene Information

Human
Gene Name:	NAE1
Uniprot:	Q13564
Entrez:	8883

Family

Belongs to:

ubiquitin-activating E1 family

Alternative Names

A-116A10.1; amyloid beta precursor protein binding protein 1; amyloid beta precursor protein binding protein 1, 59kDa; Amyloid beta precursor protein-binding protein 1, 59 kDa; amyloid beta precursor protein-binding protein 1, 59kD; Amyloid protein-binding protein 1; APPBP1APP-BP1; NEDD8 Activating Enzyme (APPBP1/UBA3); NEDD8 activating enzyme E1 subunit 1; NEDD8-activating enzyme E1 regulatory subunit; NEDD8-activating enzyme E1 subunit; protooncogene protein 1; Proto-oncogene protein 1; ula-1

Molecular Weight

Mass (kDA):
60.246 kDA

Genomic Context


Human

Location:	16q22.1
Sequence:	16; NC_000016.10 (66802878..66830977, complement)

Tissue Specificity

Ubiquitous in fetal tissues. Expressed throughout the adult brain.

Subcellular Localizations

Cell membrane. Colocalizes with APP in lipid rafts.

Diseases

Transient Cerebral Ischemia
Ischemia
Malignant Neoplasm Of Breast
Occlusion, Middle Cerebral Artery
Neurofibromatosis 1
Arterial Occlusion
Chickenpox
Exanthema
Malignant Neoplasms
Mammary Neoplasms

Cerebral Artery Occlusion
Transient Ischemic Attack
Cerebral Ischemia
Transient Ischemia

Pathways

Neurogenesis
Rna Interference
Conjugation

PTMs

Acetylation

Ubl conjugation

Research Areas

Apoptosis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NAE1

References

PMID: 8626687 by Chow N., et al. APP-BP1, a novel protein that binds to the carboxyl-terminal region of the amyloid precursor protein.

PMID: 10207026 by Gong L., et al. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.