This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
NCAN · Western blot design guide
NCAN Western Blot Planning Guide
Plan a NCAN Western blot around the catalog-observed 143.1 kDa band, image-backed A06700-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
NCAN Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 143.1 kDa observed | |
| Observed band | 143.1 kDa observed | |
| Gel | 8-10% | |
| Positive control | Cerebellum | |
| Negative control | Adipose tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 143.1 kDa |
| ⓘ | Localization | Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated NCAN Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebellum |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A06700-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected NCAN Western Blot Band Size?
Use the product-observed 143.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 143.1 kDa | Matches the authoritative product WB observation. |
| 143.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected NCAN appearancePlan around 143.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 143.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 143.1 kDa from UniProt O14594; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A06700-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 143.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing NCAN band
A06700-1 · NCAN
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NCAN Western blot
🧪Use Cerebellum as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Cerebellum (High)
Negative control: Adipose tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for NCAN
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced NCAN Western Blot Tips
Deeper troubleshooting and optimisation questions for NCAN, answered from its protein features.
Which band should guide the blot?
Use 143.1 kDa, the observation attached to the authoritative A06700-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 143.1 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adipose tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for NCAN Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A06700-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
NCAN Western Blot Reagents
Human/Mouse/Rat-reactive NCAN Western blot reagents with authoritative product imagery.
Real WB data
Anti-Neurocan/NCAN Antibody Picoband®
Cat # A06700-1
Real WB data
Anti-Neurocan/NCAN Antibody Picoband®
Cat # A06700-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O14594
- Human Protein Atlas — NCAN
- A06700-1 product record
- PMC5739734 — Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes (Oncotarget, 2017)
- PMC11098688 — Astrocyte-secreted neurocan controls inhibitory synapse formation and function (Neuron, 2024)
- PMC10805400 — Electroacupuncture promotes the repair of the damaged spinal cord in mice by mediating neurocan-perineuronal net (CNS neuroscience & therapeutics, 2024)