NNMT Antibodies

AND NNMT ELISA kits, NNMT Proteins

Many biological assays use antibodies to detect NNMT, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NNMT in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NNMT antibodies...

We validate the specificity of these antibodies to NNMT by testing them on tissues known to express NNMT positively and negatively. Browse below to find the NNMT antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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NNMT Infographic by Boster Bio

All NNMT Products

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Anti-NNMT Antibody

Western blot (WB) analysis of NNMT pAb at 1:1000 dilution
Lane1:The liver tissue lysate of Mouse(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:SK-OVCAR3 whole cell lysate(40ug)

Human, Mouse

$405
Cat # A02676
100ul

Mouse Nnmt Recombinant Protein

SDS-PAGE

$250
Cat # PROTO55239
Starting from 4ug

Human Nicotinamide N-Methyltransferase/NNMT Recombinant Protein

SDS-PAGE

$250
Cat # PROTP40261
Starting from 20ug

Anti-NNMT Mouse Monoclonal Antibody [Clone ID: OTI3D8]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody

Human, Mouse, Rat

Flow Cytometry, IF, IHC, WB

$523
Cat # M02676
100 µl

Anti-NNMT Antibody (Center)

NNMT Antibody (Center) western blot analysis in HT29 cell line lysates (35ug/lane)

Human

IHC-P, WB

$299
Cat # A02676-1
80 µl

NNMT (NM_006169) Human Over-expression Lysate

Human

$628
Cat # LS004837
20 µg, 100 µg

Anti-NNMT Antibody Picoband®

Western blot analysis of NNMT using anti-NNMT antibody (A02676-2)

IF analysis of NNMT using anti-NNMT antibody (A02676-2)

Immunoprecipitating (IP) NNMT in Hela whole cell lysate

Human, Mouse, Rat

ELISA, IP, IF, ICC, WB

$370
Cat # A02676-2
100 µg/vial

NNMT Overview

Facts about Nicotinamide N-methyltransferase.

NNMT 3D structure. Source: alphafold

Nicotinamide N-methyltransferase (NNMT)

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Gene Information

Human
Gene Name:	NNMT
Uniprot:	P40261
Entrez:	4837

Family

Belongs to:

class I-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.1; Nicotinamide NMethyltransferase; Nicotinamide N-Methyltransferase; NNMT

Molecular Weight

Mass (kDA):
29.574 kDA

Genomic Context


Human

Location:	11q23.2
Sequence:	11; NC_000011.10 (114257806..114313536)

Tissue Specificity

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Subcellular Localizations

Cytoplasm.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Parkinson Disease
5,10-methylenetetrahydrofolate Reductase Deficiency
Hyperhomocysteinemia
Neoplasm Metastasis
Renal Cell Carcinoma
Liver Carcinoma
Kidney Neoplasm
Chronic Obstructive Airway Disease
Carcinoma, Ehrlich Tumor
Malignant Neoplasm Of Breast

Lung Diseases
Lung Diseases, Obstructive
Mouth Neoplasms
Malignant Squamous Cell Neoplasm
Leukemia
Sarcoma
Malignant Neoplasm Of Urinary Bladder

Pathways

Methylation
Pathogenesis
Cell Death
Cell Migration
Brain Development
Aging
Cell Proliferation
Dna Repair
Localization
Excretion
Cell Adhesion
Transposition
Cell Activation

Programmed Cell Death
Gene Silencing
Proteolysis
Leukocyte Activation
Swimming
Fermentation
Translation

PTMs

Methylation
Demethylation

Phosphorylation
Reduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NNMT

References

PMID: 8182091 by Aksoy S., et al. Human liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization.

PMID: 8575745 by Aksoy S., et al. Human nicotinamide N-methyltransferase gene: molecular cloning, structural characterization and chromosomal localization.