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- Table of Contents
Designing a Western blot for Nucleolar protein 56? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NOP56 Western Blot GuideFacts about Nucleolar protein 56.
Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs. .
| Human | |
|---|---|
| Gene Name: | NOP56 |
| Uniprot: | O00567 |
| Entrez: | 10528 |

| Belongs to: |
|---|
| NOP5/NOP56 family |

NOL5A; NOP56 ribonucleoprotein homolog (yeast); nucleolar protein 56; nucleolar protein 5A (56kD with KKE/D repeat); nucleolar protein 5A (56kDa with KKE/D repeat); Nucleolar protein 5A
Mass (kDA):
66.05 kDA

| Human | |
|---|---|
| Location: | 20p13 |
| Sequence: | 20; NC_000020.11 (2652632..2658393) |
Nucleus, nucleolus. Cytoplasm. Nucleus, nucleoplasm.




PMID: 9372940 by Gautier T., et al. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
PMID: 12417735 by Watkins N.J., et al. Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP.