NOSIP Antibodies

AND NOSIP ELISA kits, NOSIP Proteins

Many biological assays use antibodies to detect NOSIP, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NOSIP in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NOSIP antibodies...

We validate the specificity of these antibodies to NOSIP by testing them on tissues known to express NOSIP positively and negatively. Browse below to find the NOSIP antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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NOSIP Infographic by Boster Bio

All NOSIP Products

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Specificity

Applications

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Anti-NOSIP Antibody Picoband®

Western blot analysis of NOSIP using anti-NOSIP antibody (A07558-2)

IF analysis of NOSIP using anti-NOSIP antibody (A07558-2)

IF analysis of NOSIP using anti-NOSIP antibody (A07558-2) and Anti-Beta Tubulin Antibody (M01857-3)

Human

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A07558-2
100 µg/vial

Anti-NOSIP Antibody

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night

Human, Mouse

ELISA, WB

$380
Cat # A07558-1
100ul

Anti-NOSIP Antibody

Western blot analysis of extracts of various cell lines, using NOSIP antibody at 1:1000 dilution

Human

$405
Cat # A07558
100ul

NOSIP (NM_015953) Human Over-expression Lysate

Human

$628
Cat # LS051070
20 µg, 100 µg

NOSIP Overview

Facts about Nitric oxide synthase-interacting protein.

NOSIP 3D structure. Source: alphafold

Nitric oxide synthase-interacting protein (NOSIP)

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity).

Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813). .

Gene Information

Human
Gene Name:	NOSIP
Uniprot:	Q9Y314
Entrez:	51070

Family

Belongs to:

NOSIP family

Alternative Names

CGI-25; eNOS interacting protein; eNOS-interacting protein; nitric oxide synthase interacting protein; nitric oxide synthase-interacting protein

Molecular Weight

Mass (kDA):
33.172 kDA

Genomic Context


Human

Location:	19q13.33
Sequence:	19; NC_000019.10 (49555468..49580572, complement)

Tissue Specificity

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).

Subcellular Localizations

Cytoplasm. Nucleus. Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).

Diseases

Atherosclerosis
Arteriosclerosis
Inflammation
Coronary Artery Disease
Hypertensive Disease
Coronary Heart Disease
Cardiovascular Diseases
Nervousness
Heart Failure
Vascular Diseases
Essential Hypertension
Brain Ischemia

Hepatitis, Alcoholic
Spinal Cord Injuries
Impotence, Vasculogenic
Trauma, Nervous System
Impotence, Arteriogenic
Hypokinesia
Tumor Angiogenesis

Pathways

Localization
Cellular Localization
Pathogenesis
Secretion
Cell Adhesion
Vasodilation
Cell Proliferation
Hypersensitivity
Nitric Oxide Homeostasis
Nuclear Export

Cell Death
Cell Cycle
Angiogenesis
Rna Interference
Nuclear Import

PTMs

Phosphorylation

Acylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NOSIP

References

PMID: 11149895 by Dedio J., et al. NOSIP, a novel modulator of endothelial nitric oxide synthase activity.

PMID: 15548660 by Dreyer J., et al. Nitric oxide synthase (NOS)-interacting protein interacts with neuronal NOS and regulates its distribution and activity.