Anti-NOSIP Antibody Picoband™

Product Name

Anti-NOSIP Antibody Picoband™

View all NOSIP Antibodies

SKU/Catalog Number

A07558-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOSIP Antibody Picoband™ catalog # A07558-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NOSIP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07558-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NOSIP recombinant protein (Position: M1-R278).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07558-2 is reactive to NOSIP in Human

Applications

A07558-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

33.172kDa

Background of NOSIP

Nitric oxide synthase-interacting protein is an enzyme that in humans is encoded by the NOSIP gene. The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NOSIP using anti-NOSIP antibody (A07558-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOSIP antigen affinity purified polyclonal antibody (Catalog # A07558-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOSIP at approximately 35 kDa. The expected band size for NOSIP is at 35 kDa.

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Figure 2. IF analysis of NOSIP using anti-NOSIP antibody (A07558-2).
NOSIP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOSIP Antibody (A07558-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 594 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For NOSIP (Source: Uniprot.org, NCBI)

Alternative Names

CGI-25; eNOS interacting protein; eNOS-interacting protein; nitric oxide synthase interacting protein; nitric oxide synthase-interacting protein NOSIP CGI-25 nitric oxide synthase interacting protein nitric oxide synthase-interacting protein|E3 ubiquitin-protein ligase NOSIP|RING-type E3 ubiquitin transferase NOSIP|eNOS-interacting protein

For more info on NOSIP, check out the NOSIP Infographic

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In this infographic, you will see the following information for NOSIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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