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- Table of Contents
NSD2 · Western blot design guide
NSD2 Western Blot Planning Guide
Plan a NSD2 Western blot around the catalog-observed 152.3 kDa band, image-backed A32441-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
NSD2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 152.3 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Cervix | |
| Negative control | Soft tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 152.3 kDa |
| ⓘ | Localization | Nucleus / Chromosome |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated NSD2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A32441-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected NSD2 Western Blot Band Size?
Use the product-observed 152.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 152.3 kDa | Matches the authoritative product WB observation. |
| 152.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected NSD2 appearancePlan around 152.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 152.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 152.3 kDa from UniProt O96028; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A32441-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 152.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing NSD2 band
A32441-1 · NSD2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for NSD2 Western blot
🧪Use Cervix as the first positive-control candidate and Soft tissue as the HPA Not detected negative candidate.
Positive control: Cervix (High)
Negative control: Soft tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for NSD2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Soft tissue | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced NSD2 Western Blot Tips
Deeper troubleshooting and optimisation questions for NSD2, answered from its protein features.
Which band should guide the blot?
Use 152.3 kDa, the observation attached to the authoritative A32441-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 152.3 kDa expectation.
Which positive control should I start with?
Start with Cervix, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Soft tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for NSD2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A32441-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
NSD2 Western Blot Reagents
Human-reactive NSD2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-WHSC1/NSD2 Antibody Picoband®
Cat # A32441-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O96028
- Human Protein Atlas — NSD2
- A32441-1 product record
- PMC13370883 — Aryl Aldehyde-Anchored Small Molecules Recruit FBXO22 for Targeted Degradation of NSD2 (Journal of medicinal chemistry, 2026)
- PMC9262605 — H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis (Nucleic acids research, 2022)
- PMC10116495 — Discovery of a Potent and Selective Targeted NSD2 Degrader for the Reduction of H3K36me2 (Journal of the American Chemical Society, 2023)
