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- Table of Contents
Designing an IHC experiment for NUDT21? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the NUDT21 IHC GuideFacts about Cleavage and polyadenylation specificity factor subunit 5.
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| Human | |
|---|---|
| Gene Name: | NUDT21 |
| Uniprot: | O43809 |
| Entrez: | 11051 |

| Belongs to: |
|---|
| Nudix hydrolase family |

CFIm25; CFIM25CPSF 25 kDa subunit; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specific factor 5, 25 kDa; Cleavage and polyadenylation specificity factor 25 kDa subunit; cleavage and polyadenylation specificity factor subunit 5; CPSF25; CPSF5DKFZp686H1588; Nucleoside diphosphate-linked moiety X motif 21; nudix (nucleoside diphosphate linked moiety X)-type motif 21; Nudix motif 21; pre-mRNA cleavage factor Im (25kD); Pre-mRNA cleavage factor Im 25 kDa subunit; pre-mRNA cleavage factor Im, 25kD subunit
Mass (kDA):
26.227 kDA

| Human | |
|---|---|
| Location: | 16q13 |
| Sequence: | 16; NC_000016.10 (56429133..56451332, complement) |
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763).





PMID: 9659921 by Rueegsegger U., et al. Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits.
PMID: 8626397 by Rueegsegger U., et al. Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors.