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- Table of Contents
Nsun2 Antibodies
AND Nsun2 ELISA kits, Nsun2 Proteins
Many biological assays use antibodies to detect Nsun2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Nsun2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Nsun2 antibodies...
Designing an IHC experiment for Nsun2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the NSUN2 IHC GuideNsun2 Infographic by Boster Bio
All Nsun2 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A03878-1
100 µg/vial
Cat # A03878-1
100 µg/vial
Human, Monkey, Mouse
IP, IF, IHC, ICC, WB
$370
Cat # M03878
100 μl/vial
Cat # M03878
100 μl/vial
Nsun2 Overview
Facts about RNA cytosine C(5)-methyltransferase NSUN2.
RNA cytosine C(5)-methyltransferase NSUN2 (Nsun2)
RNA methyltransferase that methylates tRNAs, and maybe RNA polymerase III transcripts. Methylates cytosine to 5- methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and in positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity).
May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
Gene Information
| Mouse | |
|---|---|
| Gene Name: | Nsun2 |
| Uniprot: | Q1HFZ0 |
| Entrez: | 28114 |
Family
| Belongs to: |
|---|
| class I-like SAM-binding methyltransferase superfamily |
Alternative Names
EC 2.1.1; EC 2.1.1.29; FLJ20303,5-methycytoisine methyltransferase; hTrm4; member 2; Myc-induced SUN-domain-containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family member 2; NOP2/Sun domain family, member 2; SAKIMisu; Substrate of AIM1/Aurora kinase B; TRM4MISU; tRNA (cytosine-5-)-methyltransferase NSUN2; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog
Molecular Weight
Mass (kDA):
85.452 kDA
Genomic Context
| Mouse | |
|---|---|
| Location: | 13 C1|13 35.55 cM |
| Sequence: | 13; |
Tissue Specificity
Ubiquitously expressed at low level. Up- regulated in tumors.
Diseases
- Malignant Neoplasms
- Neoplasms
- Distal Muscular Dystrophies
- Liver Diseases
- Hepatitis, Chronic
- Malignant Neoplasm Of Breast
- Hepatitis C, Chronic
- Malignant Tumor Of Colon
- Hypoxia
- Aneuploidy
- Hereditary Hemochromatosis
- Congenital Anomaly Of Face
Pathways
- Methylation
- Mitosis
- Interphase
- Cell Proliferation
- Rna Methylation
- Translation
- Cell Cycle
- Rna Processing
- Spindle Assembly
- Cell Adhesion
- Stem Cell Differentiation
- G2 Phase
- Trna Methylation
PTMs
- Methylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Nsun2
bosterbio.com/bosterbio-gene-info-cards/Nsun2
References
PMID: 16713953 by Frye M., et al. The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.
PMID: 19596847 by Hussain S., et al. The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability.