Nucleobindin-1 Antibodies

AND NUCB1 ELISA kits, Nucleobindin-1 Proteins

Many biological assays use antibodies to detect Nucleobindin-1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Nucleobindin-1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Nucleobindin-1 antibodies...

We validate the specificity of these antibodies to Nucleobindin-1 by testing them on tissues known to express NUCB1 positively and negatively. Browse below to find the NUCB1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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NUCB1 Infographic by Boster Bio

All NUCB1 Products

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Anti-Nucleobindin-1 NUCB1 Antibody

Western blot analysis of extracts of various cell lines, using NUCB1 antibody at 1:3000 dilution

Immunofluorescence analysis of L929 cells using NUCB1 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of U-2 OS cells using NUCB1 Polyclonal Antibody at dilution of 1:100

Human, Mouse, Rat

IF, IHC, ICC, WB

$405
Cat # A07305
100ul

Anti-Nucleobindin 1 (NUCB1) Mouse Monoclonal Antibody [Clone ID: OTI1A5]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody

Human, Mouse, Rat

Flow Cytometry, IF, IHC, WB

$523
Cat # M07305
100 µl

Nucleobindin 1 (NUCB1) (NM_006184) Human Over-expression Lysate

Human

$628
Cat # LS004924
20 µg, 100 µg

Anti-NUCB1 Antibody

Human, Mouse

ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # A07305-2
100 μl/vial

NUCB1 Overview

Facts about Nucleobindin-1.

NUCB1 3D structure. Source: alphafold

Nucleobindin-1 (NUCB1)

Major calcium-binding protein of the Golgi.
May have a role in calcium homeostasis (By similarity).

. .

Gene Information

Human
Gene Name:	NUCB1
Uniprot:	Q02818
Entrez:	4924

Family

Belongs to:

nucleobindin family

Alternative Names

DKFZp686A15286; FLJ40471; NUCCALNUC; nucleobindin 1; nucleobindin-1

Molecular Weight

Mass (kDA):
53.879 kDA

Genomic Context


Human

Location:	19q13.33
Sequence:	19; NC_000019.10 (48900312..48923372)

Tissue Specificity

Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.

Subcellular Localizations

Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Lumenal side. Cytoplasm. Secreted. A small fraction of the protein may be cytoplasmic.

Diseases

Diabetes Mellitus
Autoimmunity
Diabetes Mellitus, Non-insulin-dependent
Multiple Pregnancy
Metabolic Diseases
Obesity
Salivary Gland Diseases

Pituitary Diseases
Tissue Adhesions

Pathways

Transport
Localization
Golgi Localization
Cell Adhesion
Egg Activation
Cell Proliferation
Vesicle Transport
Amyloid Fibril Formation
Insulin Secretion
Protein Maturation

Endocytosis
Ovulation
Energy Homeostasis
Secretion

PTMs

Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NUCB1

References

PMID: 1520323 by Miura K., et al. Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure.

PMID: 8661046 by Miura K., et al. Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4.