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- Table of Contents
PARN · Western blot design guide
PARN Western Blot Planning Guide
Plan a PARN Western blot around the catalog-observed 73.5 kDa band, image-backed A01501-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PARN Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 73.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Breast | |
| Negative control | Soft tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 73.5 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PARN Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01501-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PARN Western Blot Band Size?
Use the product-observed 73.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 73.5 kDa | Matches the authoritative product WB observation. |
| 73.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PARN appearancePlan around 73.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 73.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 73.5 kDa from UniProt O95453; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01501-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 73.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PARN band
A01501-2 · PARN
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PARN Western blot
🧪Use Breast as the first positive-control candidate and Soft tissue as the HPA Not detected negative candidate.
Positive control: Breast (High)
Negative control: Soft tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PARN
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Soft tissue | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced PARN Western Blot Tips
Deeper troubleshooting and optimisation questions for PARN, answered from its protein features.
Which band should guide the blot?
Use 73.5 kDa, the observation attached to the authoritative A01501-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 73.5 kDa expectation.
Which positive control should I start with?
Start with Breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Soft tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PARN Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01501-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PARN Western Blot Reagents
Human/Mouse/Rat-reactive PARN Western blot reagents with authoritative product imagery.
Real WB data
Anti-PARN Antibody Picoband®
Cat # A01501-2
Real WB data
Anti-PARN Antibody Picoband® (monoclonal, 11D8)
Cat # M01501
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O95453
- Human Protein Atlas — PARN
- A01501-2 product record
- PMC4811377 — A Specialized Mechanism of Translation Mediated by FXR1a-Associated MicroRNP in Cellular Quiescence (Molecular cell, 2016)
- PMC5389690 — Poly(A)-specific ribonuclease regulates the processing of small-subunit rRNAs in human cells (Nucleic acids research, 2017)
- PMC6609912 — Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models (EMBO molecular medicine, 2019)
