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- Table of Contents
PBK · Western blot design guide
PBK Western Blot Planning Guide
Plan a PBK Western blot around the catalog-observed 36.1 kDa band, image-backed PB9310 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PBK Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 36.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Testis | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 36.1 kDa |
| ⓘ | Localization | Not reported |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PBK Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Testis |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9310 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PBK Western Blot Band Size?
Use the product-observed 36.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 36.1 kDa | Matches the authoritative product WB observation. |
| 36.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PBK appearancePlan around 36.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 36.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 36.1 kDa from UniProt Q96KB5; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9310. |
| Situation | Likely cause | Next action |
|---|---|---|
| 36.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PBK band
PB9310 · PBK
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PBK Western blot
🧪Use Testis as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Testis (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PBK
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced PBK Western Blot Tips
Deeper troubleshooting and optimisation questions for PBK, answered from its protein features.
Which band should guide the blot?
Use 36.1 kDa, the observation attached to the authoritative PB9310 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 36.1 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PBK Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9310 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC9355941, PMC5841183, PMC6379381.
Boster reagents
PBK Western Blot Reagents
Human/Mouse/Rat-reactive PBK Western blot reagents with authoritative product imagery.
Real WB data
Anti-PBK Antibody Picoband®
Cat # PB9310
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q96KB5
- Human Protein Atlas — PBK
- PB9310 product record
- PMC9355941 — PBK drives PARP inhibitor resistance through the TRIM37/NFκB axis in ovarian cancer.
- PMC5841183 — Enhanced Expression of the Key Mitosis Regulator Cyclin B1 Is Mediated by PDZ-Binding Kinase in Islets of Pregnant Mice.
- PMC6379381 — PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma.