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- Table of Contents
PDCD1 · Western blot design guide
PDCD1 Western Blot Planning Guide
Plan a PDCD1 Western blot around the catalog-observed 31.6 kDa band, image-backed A00178 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PDCD1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 31.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Tonsil | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 31.6 kDa |
| ⓘ | Localization | Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Mouse / Rat |
Section 1
Real Curated PDCD1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Tonsil |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00178 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PDCD1 Western Blot Band Size?
Use the product-observed 31.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 31.6 kDa | Matches the authoritative product WB observation. |
| 31.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PDCD1 appearancePlan around 31.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 31.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 31.6 kDa from UniProt Q15116; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00178. |
| Situation | Likely cause | Next action |
|---|---|---|
| 31.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PDCD1 band
A00178 · PDCD1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PDCD1 Western blot
🧪Use Tonsil as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Tonsil (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PDCD1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Tonsil | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced PDCD1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PDCD1, answered from its protein features.
Which band should guide the blot?
Use 31.6 kDa, the observation attached to the authoritative A00178 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 31.6 kDa expectation.
Which positive control should I start with?
Start with Tonsil, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PDCD1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00178 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC11496882, PMC4700833, PMC9578978.
Boster reagents
PDCD1 Western Blot Reagents
Mouse/Rat-reactive PDCD1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-PD-1/PDCD1 Antibody Picoband®
Cat # A00178
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q15116
- Human Protein Atlas — PDCD1
- A00178 product record
- PMC11496882 — A splicing isoform of PD-1 promotes tumor progression as a potential immune checkpoint.
- PMC4700833 — Melanoma Cell-Intrinsic PD-1 Receptor Functions Promote Tumor Growth.
- PMC9578978 — Upregulation of programmed cell death 1 by interferon gamma and its biological functions in human monocytes.