POLD3 Antibodies

AND POLD3 ELISA kits, POLD3 Proteins

Many biological assays use antibodies to detect POLD3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with POLD3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop POLD3 antibodies...

We validate the specificity of these antibodies to POLD3 by testing them on tissues known to express POLD3 positively and negatively. Browse below to find the POLD3 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for POLD3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the POLD3 Western Blot Guide

POLD3 Infographic by Boster Bio

All POLD3 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-POLD3 Antibody Picoband®

Western blot analysis of POLD3 using anti-POLD3 antibody (A05111-2)

Human, Mouse, Rat

ELISA, WB

$370
Cat # A05111-2
100 µg/vial

Anti-POLD3 Antibody

Western Blot analysis of hela cells using DNA pol δ3 Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from LOVO, HepG2, and mouse brain cells, using POLD3 Antibody

Human, Mouse, Rat

ELISA, WB

$335
Cat # A30646
100ul

POLD3 Overview

Facts about DNA polymerase delta subunit 3.

POLD3 3D structure. Source: alphafold

DNA polymerase delta subunit 3 (POLD3)

As a part of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a part in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity.

They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433).

Gene Information

Human
Gene Name:	POLD3
Uniprot:	Q15054
Entrez:	10714

Family

Belongs to:

No superfamily

Alternative Names

DNA polymerase delta subunit 3; DNA polymerase delta subunit p66; DNA polymerase delta, subunit 3; KIAA0039MGC119643; MGC119642; P66; P68; polymerase (DNA directed), delta 3; polymerase (DNA-directed), delta 3, accessory subunit

Molecular Weight

Mass (kDA):
51.4 kDA

Genomic Context


Human

Location:	11q13.4
Sequence:	11; NC_000011.10 (74592582..74669341)

Subcellular Localizations

Cytoplasm. Nucleus. Partially colocalizes with PCNA and POLD1 at S phase replication sites (PubMed:11595739). Recruited to DNA damage sites within 2 hours following UV irradiation (PubMed:20227374, PubMed:22801543).

Diseases

Malignant Neoplasms
Neoplasms
Colorectal Neoplasms
Colorectal Cancer
Cell Invasion
Cell Transformation, Neoplastic
Mutation, Out-of-frame
Tumor Progression
Malignant Neoplasm Of Breast

Malignant Paraganglionic Neoplasm
Microsatellite Instability
Carcinogenesis
Mammary Neoplasms

Interacting Proteins

PCNA
POLD2

Pathways

Dna Replication
Dna Repair
Cell Cycle
Mismatch Repair
Cell Migration
Translesion Synthesis
S Phase

Localization
Nuclear Import

PTMs

Ubiquitination

Phosphorylation

Research Areas

Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLD3

References

PMID: 10219083 by Hughes P., et al. Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts.

PMID: 10852724 by Mo J.-Y., et al. Evidence that DNA polymerase delta isolated by immunoaffinity chromatography exhibits high-molecular weight characteristics and is associated with the KIAA0039 protein and RPA.