This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
POLI · Western blot design guide
POLI Western Blot Planning Guide
Plan a POLI Western blot around the catalog-observed 83 kDa band, image-backed A01376-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
POLI Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 83 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Parathyroid gland | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 83 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated POLI Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Parathyroid gland |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01376-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected POLI Western Blot Band Size?
Use the product-observed 83 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 83 kDa | Matches the authoritative product WB observation. |
| 83 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected POLI appearancePlan around 83 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 83 kDa; use this as the primary experimental expectation. |
| Calculated mass | 83 kDa from UniProt Q9UNA4; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01376-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 83 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing POLI band
A01376-1 · POLI
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for POLI Western blot
🧪Use Parathyroid gland as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Parathyroid gland (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for POLI
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Parathyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Thyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced POLI Western Blot Tips
Deeper troubleshooting and optimisation questions for POLI, answered from its protein features.
Which band should guide the blot?
Use 83 kDa, the observation attached to the authoritative A01376-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 83 kDa expectation.
Which positive control should I start with?
Start with Parathyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for POLI Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01376-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
POLI Western Blot Reagents
Human/Mouse/Rat-reactive POLI Western blot reagents with authoritative product imagery.
Real WB data
Anti-DNA Polymerase iota/POLI Antibody Picoband®
Cat # A01376-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UNA4
- Human Protein Atlas — POLI
- A01376-1 product record
- PMC13141746 — Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer (Translational oncology, 2026)
- PMC10412619 — Polymerase iota (POLI) confers radioresistance of esophageal squamous cell carcinoma by regulating RAD51 stability and facilitating homologous recombination (Cell death discovery, 2023)
- PMC9284323 — Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H(2)O(2) Sensitivity in POLI-Deficient Cells (Chemical research in toxicology, 2020)
