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- Table of Contents
PON2 · Western blot design guide
PON2 Western Blot Planning Guide
Plan a PON2 Western blot around the catalog-observed 39.4 kDa band, image-backed A02027-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PON2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 39.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Caudate | |
| Negative control | Salivary gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 39.4 kDa |
| ⓘ | Localization | Membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated PON2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02027-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PON2 Western Blot Band Size?
Use the product-observed 39.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 39.4 kDa | Matches the authoritative product WB observation. |
| 39.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PON2 appearancePlan around 39.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 39.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 39.4 kDa from UniProt Q15165; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02027-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 39.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PON2 band
A02027-2 · PON2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PON2 Western blot
🧪Use Caudate as the first positive-control candidate and Salivary gland as the HPA Not detected negative candidate.
Positive control: Caudate (High)
Negative control: Salivary gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PON2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced PON2 Western Blot Tips
Deeper troubleshooting and optimisation questions for PON2, answered from its protein features.
Which band should guide the blot?
Use 39.4 kDa, the observation attached to the authoritative A02027-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 39.4 kDa expectation.
Which positive control should I start with?
Start with Caudate, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Salivary gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PON2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02027-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC2790986, PMC8084648, PMC4016019.
Boster reagents
PON2 Western Blot Reagents
Human-reactive PON2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-PON2 Antibody Picoband®
Cat # A02027-2
Real WB data
Anti-PON2 Antibody Picoband®
Cat # PB9779
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q15165
- Human Protein Atlas — PON2
- A02027-2 product record
- PMC2790986 — A common mutation in paraoxonase-2 results in impaired lactonase activity.
- PMC8084648 — Fisetin Alleviates Neointimal Hyperplasia via PPARγ/PON2 Antioxidative Pathway in SHR Rat Artery Injury Model.
- PMC4016019 — Paraoxonase 2 protein is spatially expressed in the human placenta and selectively reduced in labour.