This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Liprin-alpha-1.
.
Human | |
---|---|
Gene Name: | PPFIA1 |
Uniprot: | Q13136 |
Entrez: | 8500 |
Belongs to: |
---|
liprin family |
FLJ42630; FLJ43474; LAR-interacting protein 1; LIP.1; LIP-1; LIP1FLJ41337; LIPRIN; Liprin-alpha1; liprin-alpha-1; MGC26800; Protein tyrosine phosphatase receptor type f polypeptide-interacting proteinalpha-1; protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interactingprotein (liprin), alpha 1; PTPRF-interacting protein alpha-1
Mass (kDA):
135.779 kDA
Human | |
---|---|
Location: | 11q13.3 |
Sequence: | 11; NC_000011.10 (70270687..70384405) |
Ubiquitous.
Cytoplasm. Colocalizes with PTPRF at the ends of focal adhesions most proximal to the cell nucleus.
The PPFIA1 gene is a protein that controls a cell's ability produce cytokines. There are three main types: type 1, type 2, and type 3. Each type has a different purpose in the lab. This article will highlight the differences between them. It also discusses the workings of PPFIA1 and its implications for cell culture experiments.
The PPFIA1 cell marker has many uses in cell biology including imaging of the cell membrane. Its red fluorescent protein (CFP) staining allows researchers to visualize the protein in live cells. The PPFIA1 gene encodes a protein that is expressed in eukaryotic cells. The gene can be expressed by many organisms, including humans. Below are the best uses for this marker.
PPFIA1 is essential for the polarization and trafficking of lipids and proteins in the cell environment. Basolateral targeting of PGCs by PPFIA1 is encouraged, even for those with a5b1 integrins. Further research will determine whether PPFIA1 activates additional biochemical signals. It is however a valuable marker for cell imaging.
Boster Biologicals products include high-sensitivity ELISA kit and IHC-optimized, polyclonal antibodies. These products have been validated against panels of 250 human cells and tissues. Boster Bio is a company that has been in existence since 1993. It focuses on immunological reagents. Its products are validated quantitatively against known amounts of recombinant proteins.
MRC Harwell bought the Liprina2 mice. They were obtained by homologous replication, crossed with Flp mice, and kept in homozygotes. Genotyping was performed using oligonucleotide primers. After the mice had been genotyped, they were crossed with conditional Liprina3 mouse. Additionally, conditional Liprina2 mice performed functionally identical to wild-type counterparts.
You've come to right place if your search for a high quality PPFIA3 antibody. Boster Bio's antibodies have been extensively tested and cited widely in the scientific community for their high-affinity as well as specificity and high-quality. They've been validated on Western Blotting, Immunohistochemistry, and ELISA, and they are compatible with a wide variety of applications.
Boster Bio specializes not only in producing high-quality antibody products, but also in the development of picogram-sensitive ELISA and IHC optimized polyclonal antibodies. Boster Bio's antibodies are quantitatively tested against recombinant protein recombinant proteins. Their high affinity and specificity translate to exceptional performance for your experiments. Boster Bio's products have been cited in over 29,000 publications. They are a long-standing company that excels in immunology.
Two polypeptides are encoded by the PPFIA1 protein gene: IL-21 & NM02130. After equilibration, a 0.15% deoxycholic-acid solution is added to the polypeptide protein. The purified polypeptide protein then undergoes chromatography on Sephadex G75 columns. The polypeptide protein is then stored at -80 degC and is ready for use.
The Boster Bio Anti–KHDRBS2 Antibody could be the right choice for you if you are looking for a new PPFIA1 indicator. This antibody reacts with Human, and has been tested for IP and IHC applications. It is available in a 100-ul Liquid form and is compatible with both IHC and WB.
Picokine(tm), a platform that offers high-sensitivity ELISA kit with picogram-level sensitivity, is available. These ELISA kits are validated for a wide variety of samples and can be purchased upon request. Picoband's polymer-based secondary antibody saves IHC time by saving 30 min in IHC. Picoband is powered through the insights of its BeNeLux distributor Sanbio and technical support from Picoband.
The PPFIA1 gene encodes the polypeptide IL-21. Several polypeptides encode IL-21. This gene can be used for drug development and discovery of novel biological agents. Moreover, the PPFIA1 gene can aid in the diagnosis of a variety of diseases and conditions. This gene may also be useful in diagnosing fibrosis.
Boster Bio stocks a variety of antibodies to detect PPFIA1. These products are designed specifically for immunohistochemistry, WB, and Flow. All of their antibodies have been tested against 250 tissues. Boster Bio also validates its products quantitatively by testing them with known amounts of recombinant protein. These studies support their high affinity and specificity.
PPFIA1 can be found in the 11q13 amplification area. This region is amplified by up to 20% breast cancers. The gene is correlated the CCND1 driver genome, and PPFIA1 gene expression levels are positively correlated CCND1 mRNA level in luminal tumors. Interestingly, high levels PPFIA1 expression are associated with poor response of tamoxifen to treatment and poor survival following endocrine therapy.
PPFIA1 gene expression was tested using immunohistochemistry on sections of tissue microarray sections measuring 4 mm. The antibody was provided by ABclonal, UK at a 1:100 dilution. TMA cores weren't evaluated in cases where the tumor burden was greater than 15% or PPFIA1 level was higher than normal. Positive staining indicated PPFIA1 expression.
The PPFIA1 proteins is an important marker for many biological process. Boster offers a line of antibodies against this protein with high-affinity, well-cited primary antibodies. Boster antibodies have been tested and validated in Immunohistochemistry, Western Blotting and ELISA, making them a trusted choice for research. We offer multiple species antibodies. Many of them are highly specific for PPFIA1.
Boster Bio's high-affinity PPFIA10 antibodies reacts with Human. You can purchase it from their catalog. It can be stored at 20°C and used for up one year. The antibody is also stored at -20degC and contains 4mg Trehalose. To maximize your antibody's sensitivity and specificity, use a blocking peptide, which is available for purchase separately.
PMID: 7796809 by Serra-Pages C., et al. The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LAR-interacting protein co-localize at focal adhesions.
PMID: 8524829 by Pulido R., et al. The LAR/PTP delta/PTP sigma subfamily of transmembrane protein- tyrosine-phosphatases: multiple human LAR, PTP delta, and PTP sigma isoforms are expressed in a tissue-specific manner and associate with the LAR-interacting protein LIP.1.