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- Table of Contents
PPP1R12A · Western blot design guide
PPP1R12A Western Blot Planning Guide
Plan a PPP1R12A Western blot around the catalog-observed 115.3 kDa band, image-backed PA1681 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PPP1R12A Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 115.3 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Breast | |
| Negative control | Soft tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 115.3 kDa |
| ⓘ | Localization | Cytoplasm / Cytoplasm, cytoskeleton, stress fiber |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PPP1R12A Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA1681 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PPP1R12A Western Blot Band Size?
Use the product-observed 115.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 115.3 kDa | Matches the authoritative product WB observation. |
| 115.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PPP1R12A appearancePlan around 115.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 115.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 115.3 kDa from UniProt O14974; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA1681. |
| Situation | Likely cause | Next action |
|---|---|---|
| 115.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PPP1R12A band
PA1681 · PPP1R12A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PPP1R12A Western blot
🧪Use Breast as the first positive-control candidate and Soft tissue as the HPA Not detected negative candidate.
Positive control: Breast (High)
Negative control: Soft tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PPP1R12A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced PPP1R12A Western Blot Tips
Deeper troubleshooting and optimisation questions for PPP1R12A, answered from its protein features.
Which band should guide the blot?
Use 115.3 kDa, the observation attached to the authoritative PA1681 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 115.3 kDa expectation.
Which positive control should I start with?
Start with Breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Soft tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PPP1R12A Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA1681 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PPP1R12A Western Blot Reagents
Human/Mouse/Rat-reactive PPP1R12A Western blot reagents with authoritative product imagery.
Real WB data
Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband®
Cat # PA1681
Real WB data
Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband®
Cat # PB9737
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O14974
- Human Protein Atlas — PPP1R12A
- PA1681 product record
- PMC10643656 — PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation (Scientific reports, 2023)
- PMC5028707 — Identification of antisense long noncoding RNAs that function as SINEUPs in human cells (Scientific reports, 2016)
- PMC3367048 — Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin (Journal of proteomics, 2012)