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- Table of Contents
PPP1R1B · Western blot design guide
PPP1R1B Western Blot Planning Guide
Plan a PPP1R1B Western blot around the catalog-observed 23 kDa band, image-backed PB9879 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PPP1R1B Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 23 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Caudate | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 23 kDa |
| ⓘ | Localization | Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PPP1R1B Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Caudate |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9879 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PPP1R1B Western Blot Band Size?
Use the product-observed 23 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 23 kDa | Matches the authoritative product WB observation. |
| 23 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PPP1R1B appearancePlan around 23 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 23 kDa; use this as the primary experimental expectation. |
| Calculated mass | 23 kDa from UniProt Q9UD71; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9879. |
| Situation | Likely cause | Next action |
|---|---|---|
| 23 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PPP1R1B band
PB9879 · PPP1R1B
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PPP1R1B Western blot
🧪Use Caudate as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Caudate (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PPP1R1B
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced PPP1R1B Western Blot Tips
Deeper troubleshooting and optimisation questions for PPP1R1B, answered from its protein features.
Which band should guide the blot?
Use 23 kDa, the observation attached to the authoritative PB9879 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 23 kDa expectation.
Which positive control should I start with?
Start with Caudate, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PPP1R1B Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9879 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PPP1R1B Western Blot Reagents
Human/Mouse/Rat-reactive PPP1R1B Western blot reagents with authoritative product imagery.
Real WB data
Anti-DARPP32/PPP1R1B Antibody Picoband®
Cat # PB9879
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UD71
- Human Protein Atlas — PPP1R1B
- PB9879 product record
- PMC6533496 — LncRNA KCNQ1OT1 enhanced the methotrexate resistance of colorectal cancer cells by regulating miR-760/PPP1R1B via the cAMP signalling pathway (Journal of cellular and molecular medicine, 2019)
- PMC7680408 — Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis (Gastroenterology, 2020)
- PMC10463655 — Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers (Journal of translational medicine, 2023)