PPP4R1 · Western blot design guide

PPP4R1 Western Blot Planning Guide

Plan a PPP4R1 Western blot around the catalog-observed 107 kDa band, image-backed A14144-2 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for PPP4R1 (PPP4R1): expected band 107 kDa, antibody A14144-2, and PMC-cited SDS-PAGE protocol steps — PPP4R1 Western blot protocol sheet — expected band 107 kDa, antibody A14144-2, controls and PMC citations. Open the full PPP4R1 WB guide →

Navigate to: Protocols Band Controls Antibodies

PPP4R1 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	107 kDa
	Observed band	Not reported — verify product WB image
	Gel	8-10%
	Positive control ⓘ	Adipose tissue
	Negative control ⓘ	Heart muscle

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	107 kDa
ⓘ	Localization	Not reported
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Section 1

Real Curated PPP4R1 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adipose tissue
Gel %	8-10%
Load	20-30 µg total protein per lane
Transfer	Wet/tank, extended transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A14144-2 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected PPP4R1 Western Blot Band Size?

Use the product-observed 107 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
107 kDa	Matches the authoritative product WB observation.
107 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected PPP4R1 appearancePlan around 107 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	107 kDa; use this as the primary experimental expectation.
Calculated mass	107 kDa from UniProt Q8TF05; retain as context.
Gel selection	8-10%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A14144-2.

Why is my band missing or off?
Situation	Likely cause	Next action
107 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q8TF05 Human Protein Atlas product datasheet (A14144-2)

Experiment evidence

Western blot images showing PPP4R1 band

A14144-2 · PPP4R1

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for PPP4R1 Western blot

🧪Use Adipose tissue as the first positive-control candidate and Heart muscle as the HPA Not detected negative candidate.

Positive control: Adipose tissue (High)

Negative control: Heart muscle (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for PPP4R1

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	Reported tissue cells	High	Protein (HPA)	HPA →
Breast	Reported tissue cells	High	Protein (HPA)	HPA →
Appendix	Reported tissue cells	High	Protein (HPA)	HPA →
Bone marrow	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Heart muscle	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced PPP4R1 Western Blot Tips

Deeper troubleshooting and optimisation questions for PPP4R1, answered from its protein features.

Which band should guide the blot?

Use 107 kDa, the observation attached to the authoritative A14144-2 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 107 kDa expectation.

Which positive control should I start with?

Start with Adipose tissue, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Heart muscle as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 8-10% consistently across the quick facts, protocol table, and poster.

What transfer method to use for PPP4R1 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A14144-2 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC10928629, PMC10111635.

Boster reagents

PPP4R1 Western Blot Reagents

Human/Mouse/Rat-reactive PPP4R1 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for PPP4R1 using A14144-2; observed band 107 kDa

Anti-PPP4R1 Antibody Picoband®

Cat # A14144-2

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.