PRKCH · Western blot design guide

PRKCH Western Blot Planning Guide

Plan a PRKCH Western blot around the catalog-observed 77.8 kDa band, image-backed PB9556 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for PRKCH (PRKCH): expected band 77.8 kDa, antibody PB9556, and PMC-cited SDS-PAGE protocol steps — PRKCH Western blot protocol sheet — expected band 77.8 kDa, antibody PB9556, controls and PMC citations. Open the full PRKCH WB guide →

Navigate to: Protocols Band Controls Antibodies

PRKCH Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	77.8 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Cervix
	Negative control ⓘ	Adipose tissue

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	77.8 kDa
ⓘ	Localization	Cytoplasm
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human

Section 1

Real Curated PRKCH Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Appendix
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	PB9556 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected PRKCH Western Blot Band Size?

Use the product-observed 77.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
77.8 kDa	Matches the authoritative product WB observation.
77.8 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected PRKCH appearancePlan around 77.8 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	77.8 kDa; use this as the primary experimental expectation.
Calculated mass	77.8 kDa from UniProt P24723; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with PB9556.

Why is my band missing or off?
Situation	Likely cause	Next action
77.8 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt P24723 Human Protein Atlas product datasheet (PB9556)

Experiment evidence

Western blot images showing PRKCH band

PB9556 · PRKCH

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for PRKCH Western blot

🧪Use Cervix as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.

Positive control: Cervix (High)

Negative control: Adipose tissue (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for PRKCH

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cervix	Reported tissue cells	High	Protein (HPA)	HPA →
Colon	Reported tissue cells	High	Protein (HPA)	HPA →
Appendix	Reported tissue cells	High	Protein (HPA)	HPA →
Bronchus	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced PRKCH Western Blot Tips

Deeper troubleshooting and optimisation questions for PRKCH, answered from its protein features.

Which band should guide the blot?

Use 77.8 kDa, the observation attached to the authoritative PB9556 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 77.8 kDa expectation.

Which positive control should I start with?

Start with Cervix, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Adipose tissue as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for PRKCH Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should PB9556 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC12399156, PMC4162097, PMC4040250.

Boster reagents

PRKCH Western Blot Reagents

Human-reactive PRKCH Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for PRKCH using PB9556; observed band 77.8 kDa

Anti-PKC eta/PRKCH Antibody Picoband®

Cat # PB9556

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.