Protein patched homolog 1 Antibodies

AND PTCH1 ELISA kits, Protein patched homolog 1 Proteins

Many biological assays use antibodies to detect Protein patched homolog 1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Protein patched homolog 1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Protein patched homolog 1 antibodies...

We validate the specificity of these antibodies to Protein patched homolog 1 by testing them on tissues known to express PTCH1 positively and negatively. Browse below to find the PTCH1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for Protein patched homolog 1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the PTCH1 IHC Guide

PTCH1 Infographic by Boster Bio

All PTCH1 Products

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Specificity

Applications

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Anti-Patched (A2) PTCH1 Antibody

Western blot (WB) analysis of Patched (A2) polyclonal antibody at 1:500 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:The Testis tissue lysate of Mouse(40ug)
Lane6:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of Patched (A2) pAb in paraffin-embedded human breast carcinoma tissue at 1:50

Human, Mouse, Rat

IHC, WB

$405
Cat # A00441
100ul

Patched Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from mouse muscle cells

Human, Mouse

ELISA

$480
Cat # EKC1441
1 kit, containing one 96-well plate and all necessary reagents

PTCH1 (NM_000264) Human Over-expression Lysate

Human

$960
Cat # LS005727
20 µg, 100 µg

PTCH1 Overview

Facts about Protein patched homolog 1.

PTCH1 3D structure. Source: alphafold

Protein patched homolog 1 (PTCH1)

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.

Appears to have a tumor suppressor function, as inactivation of this protein is most likely a necessary, if not sufficient step for tumorigenesis. .

Gene Information

Human
Gene Name:	PTCH1
Uniprot:	Q13635
Entrez:	5727

Family

Belongs to:

patched family

Alternative Names

BCNS; BCNSFLJ42602; FLJ26746; HPE7; mes; NBCCS; patched (Drosophila) homolog; Patched 1; patched homolog (Drosophila); patched homolog 1 (Drosophila); patched homolog 1; protein patched homolog 1; PTC; ptc1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH; PTCH1; PTCH11

Molecular Weight

Mass (kDA):
160.545 kDA

Genomic Context


Human

Location:	9q22.32
Sequence:	9; NC_000009.12 (95442980..95517057, complement)

Tissue Specificity

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

Neoplasms
Carcinoma
Malignant Neoplasms
Basal Cell Carcinoma
Basal Cell Nevus Syndrome
Medulloblastoma
Cell Transformation, Neoplastic
Skin Neoplasms
Malignant Paraganglionic Neoplasm
Carcinogenesis
Cerebellar Neoplasms
Brain Neoplasms
Rhabdomyosarcoma

Congenital Abnormality
Jaw Keratocyst
Neoplasm Metastasis
Odontogenic Tumors
Melanocytic Nevus
Adenocarcinoma
Malignant Squamous Cell Neoplasm

Interacting Proteins

CCNB1
GRK2
SHH
YAP1

Pathways

Cell Proliferation
Pathogenesis
Cell Cycle
Cell Growth
Methylation
Cell Death
Localization
Transport
Translation
Angiogenesis
Regeneration
Dna Repair
Secretion

Cell Differentiation
Intraflagellar Transport
Osteoblast Differentiation
Gene Silencing
Neurogenesis
Cell Migration
Oncogenesis

PTMs

Methylation
Phosphorylation
Cleavage

Demethylation
Acetylation
Dephosphorylation

Palmitoylation

Research Areas

Cell Cycle and Replication
Stem Cell Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PTCH1

References

PMID: 8658145 by Johnson R.L., et al. Human homolog of patched, a candidate gene for the basal cell nevus syndrome.

PMID: 8647801 by Hahn H., et al. A mammalian patched homolog is expressed in target tissues of sonic hedgehog and maps to a region associated with developmental abnormalities.