Polr1e Antibodies

AND Polr1e ELISA kits, Polr1e Proteins

Many biological assays use antibodies to detect Polr1e, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Polr1e in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Polr1e antibodies...

We validate the specificity of these antibodies to Polr1e by testing them on tissues known to express Polr1e positively and negatively. Browse below to find the Polr1e antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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Polr1e Infographic by Boster Bio

All Polr1e Products

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Figures

Specificity

Applications

Price

Anti-POLR1E Antibody

Western blot analysis of extracts of various cell lines, using POLR1E antibody at 1:3000 dilution

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody

Human, Mouse, Rat

IP, WB

$405
Cat # A11077
100ul

Anti-POLR1E Antibody

Immunohistochemical analysis of paraffin-embedded human brain

Human, Mouse

ELISA, IF, IHC, WB

$380
Cat # A11077-1
100ul

PRAF1 (POLR1E) (NM_022490) Human Recombinant Protein

Coomassie blue staining of purified POLR1E protein

$1249
Cat # PROTQ9GZS1
20 µg

PRAF1 (POLR1E) (NM_022490) Human Over-expression Lysate

Human

$628
Cat # LS064425
20 µg, 100 µg

Anti-POLR1E Antibody Picoband®

Western blot analysis of POLR1E using anti-POLR1E antibody (A11077-2)

IF analysis of POLR1E using anti-POLR1E antibody (A11077-2) and anti-Beta Tubulin antibody (M01857-3)

Immunoprecipitating POLR1E in RT4 whole cell lysate

Human

ELISA, IP, IF, ICC, WB

$370
Cat # A11077-2
100 μg/vial

Polr1e Overview

Facts about DNA-directed RNA polymerase I subunit RPA49.

DNA-directed RNA polymerase I subunit RPA49 (Polr1e)

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors.

Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. .

Gene Information

Mouse
Gene Name:	Polr1e
Uniprot:	Q8K202
Entrez:	64424

Family

Belongs to:

eukaryotic RPA49/POLR1E RNA polymerase subunit family

Alternative Names

DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; FLJ13390; FLJ13970; FLJ43482; PAF53RNA polymerase I-associated factor 1; polymerase (RNA) I associated factor 1; polymerase (RNA) I polypeptide E, 53kDa; PRAF1; RNA polymerase I associated factor 53; RNA polymerase I subunit A49; RNA polymerase I-associated factor 53; RP11-405L18.3

Molecular Weight

Mass (kDA):
54.034 kDA

Genomic Context


Mouse

Location:	4\|4 B1
Sequence:	4;

Diseases

Retinoblastoma
Liver Carcinoma
Liver Neoplasms, Experimental
Albinism

Pathways

Cell Cycle
Rrna Processing
Cell Cycle Arrest
Rrna Transcription

PTMs

Phosphorylation

Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Polr1e

References

PMID: 8641287 by Hanada K., et al. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription.

PMID: 9254723 by Seither P., et al. Constitutive and strong association of PAF53 with RNA polymerase I.