RANBP2 · Western blot design guide

RANBP2 Western Blot Planning Guide

Plan a RANBP2 Western blot around the catalog-observed 358.2 kDa band, image-backed A00981-1 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for RANBP2 (RANBP2): expected band 358.2 kDa, antibody A00981-1, and PMC-cited SDS-PAGE protocol steps — RANBP2 Western blot protocol sheet — expected band 358.2 kDa, antibody A00981-1, controls and PMC citations. Open the full RANBP2 WB guide →

Navigate to: Protocols Band Controls Antibodies

RANBP2 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	358.2 kDa
	Observed band	Not reported — verify product WB image
	Gel	4-12% gradient
	Positive control ⓘ	Cerebellum
	Negative control ⓘ	Target knockdown/knockout

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	358.2 kDa
ⓘ	Localization	Nucleus / Nucleus membrane
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Section 1

Real Curated RANBP2 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adrenal gland
Gel %	4-12% gradient
Load	20-30 µg total protein per lane
Transfer	Wet/tank, extended transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A00981-1 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected RANBP2 Western Blot Band Size?

Use the product-observed 358.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
358.2 kDa	Matches the authoritative product WB observation.
358.2 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected RANBP2 appearancePlan around 358.2 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	358.2 kDa; use this as the primary experimental expectation.
Calculated mass	358.2 kDa from UniProt P49792; retain as context.
Gel selection	4-12% gradient; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A00981-1.

Why is my band missing or off?
Situation	Likely cause	Next action
358.2 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt P49792 Human Protein Atlas product datasheet (A00981-1)

Experiment evidence

Western blot images showing RANBP2 band

A00981-1 · RANBP2

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for RANBP2 Western blot

🧪Use Cerebellum as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.

Positive control: Cerebellum (High)

Negative control: Target knockdown/knockout

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for RANBP2

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cerebellum	Reported tissue cells	High	Protein (HPA)	HPA →
Adrenal gland	Reported tissue cells	High	Protein (HPA)	HPA →
Appendix	Reported tissue cells	High	Protein (HPA)	HPA →
Bronchus	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source

Section 3

Advanced RANBP2 Western Blot Tips

Deeper troubleshooting and optimisation questions for RANBP2, answered from its protein features.

Which band should guide the blot?

Use 358.2 kDa, the observation attached to the authoritative A00981-1 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 358.2 kDa expectation.

Which positive control should I start with?

Start with Cerebellum, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use a target knockdown/knockout control when no HPA Not detected tissue is available.

Which gel should I use?

Use 4-12% gradient consistently across the quick facts, protocol table, and poster.

What transfer method to use for RANBP2 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A00981-1 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC11898759, PMC12817083.

Boster reagents

RANBP2 Western Blot Reagents

Human/Mouse/Rat-reactive RANBP2 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for RANBP2 using A00981-1; observed band 358.2 kDa

Anti-RANBP2 Antibody Picoband®

Cat # A00981-1

View product →

Real WB data Western blot validation image for RANBP2 using A00981; observed band 358.2 kDa

Anti-RanBP2 Antibody Picoband®

Cat # A00981