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- Table of Contents
RASA1 · Western blot design guide
RASA1 Western Blot Planning Guide
Plan a RASA1 Western blot around the catalog-observed 116.4 kDa band, image-backed PB9796 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
RASA1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 116.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Fallopian tube | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 116.4 kDa |
| ⓘ | Localization | Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated RASA1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bronchus |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9796 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected RASA1 Western Blot Band Size?
Use the product-observed 116.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 116.4 kDa | Matches the authoritative product WB observation. |
| 116.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected RASA1 appearancePlan around 116.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 116.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 116.4 kDa from UniProt P20936; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9796. |
| Situation | Likely cause | Next action |
|---|---|---|
| 116.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing RASA1 band
PB9796 · RASA1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RASA1 Western blot
🧪Use Fallopian tube as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Fallopian tube (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for RASA1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced RASA1 Western Blot Tips
Deeper troubleshooting and optimisation questions for RASA1, answered from its protein features.
Which band should guide the blot?
Use 116.4 kDa, the observation attached to the authoritative PB9796 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 116.4 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for RASA1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9796 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
RASA1 Western Blot Reagents
Human-reactive RASA1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-RASA1 Antibody Picoband®
Cat # PB9796
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P20936
- Human Protein Atlas — RASA1
- PB9796 product record
- PMC6856583 — Long non-coding RNA LOC554202 promotes acquired gefitinib resistance in non-small cell lung cancer through upregulating miR-31 expression (Journal of Cancer, 2019)
- PMC6949091 — CircAHNAK1 inhibits proliferation and metastasis of triple-negative breast cancer by modulating miR-421 and RASA1 (Aging, 2019)
- PMC5029671 — Inactivation of RASA1 promotes melanoma tumorigenesis via R-Ras activation (Oncotarget, 2016)
