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- Table of Contents
ROCK1 · Western blot design guide
ROCK1 Western Blot Planning Guide
Plan a ROCK1 Western blot around the catalog-observed 158.2 kDa band, image-backed A00722-6 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ROCK1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 158.2 kDa observed | |
| Observed band | 158.2 kDa observed | |
| Gel | 8-10% | |
| Positive control | Fallopian tube | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 158.2 kDa |
| ⓘ | Localization | Cytoplasm / Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated ROCK1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00722-6 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ROCK1 Western Blot Band Size?
Use the product-observed 158.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 158.2 kDa | Matches the authoritative product WB observation. |
| 158.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ROCK1 appearancePlan around 158.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 158.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 158.2 kDa from UniProt Q13464; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00722-6. |
| Situation | Likely cause | Next action |
|---|---|---|
| 158.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ROCK1 band
A00722-6 · ROCK1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ROCK1 Western blot
🧪Use Fallopian tube as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Fallopian tube (High)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ROCK1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced ROCK1 Western Blot Tips
Deeper troubleshooting and optimisation questions for ROCK1, answered from its protein features.
Which band should guide the blot?
Use 158.2 kDa, the observation attached to the authoritative A00722-6 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 158.2 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ROCK1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00722-6 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ROCK1 Western Blot Reagents
Human/Mouse/Rat-reactive ROCK1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-ROCK1 Antibody Picoband®
Cat # A00722-6
Real WB data
Anti-ROCK1 Antibody Picoband®
Cat # A00722-4
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13464
- Human Protein Atlas — ROCK1
- A00722-6 product record
- PMC9535064 — Rho-associated kinase1 promotes laryngeal squamous cell carcinoma tumorigenesis and progression via the FAK signaling pathway (Discover oncology, 2022)
- PMC5089992 — ROCK1 Is Associated with Alzheimer's Disease-Specific Plaques, as well as Enhances Autophagosome Formation But not Autophagic Aβ Clearance (Frontiers in cellular neuroscience, 2016)
- PMC5849190 — Disruption of ROCK1 gene restores autophagic flux and mitigates doxorubicin-induced cardiotoxicity (Oncotarget, 2018)