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- Table of Contents
RPA1 Antibodies
AND RPA1 ELISA kits, RPA1 Proteins
Many biological assays use antibodies to detect RPA1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RPA1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RPA1 antibodies...
Designing a Western blot for RPA1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the RPA1 Western Blot GuideDesigning an IHC experiment for RPA1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the RPA1 IHC GuideRPA1 Infographic by Boster Bio
All RPA1 Products
Product
Figures
Specificity
Applications
Price
Human, Monkey, Mouse
Flow Cytometry, IF, IHC, WB
$370
Cat # M01317-2
100 μg/vial
Cat # M01317-2
100 μg/vial
Human, Rat
Flow Cytometry, IF, ICC, WB
$370
Cat # M01324-2
100 μg/vial
Cat # M01324-2
100 μg/vial
Human, Rat
Flow Cytometry, IF, ICC, WB
$370
Cat # M01324-1
100 μg/vial
Cat # M01324-1
100 μg/vial
Human
Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # M01317-1
100 μl
Cat # M01317-1
100 μl
RPA1 Overview
Facts about Replication protein A 70 kDa DNA-binding subunit.
Replication protein A 70 kDa DNA-binding subunit (RPA1)
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different complexes and proteins involved in DNA metabolism (PubMed:27723720, PubMed:27723717).
Thereby, it plays a vital role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.
Gene Information
| Human | |
|---|---|
| Gene Name: | RPA1 |
| Uniprot: | P27694 |
| Entrez: | 6117 |
Family
| Belongs to: |
|---|
| replication factor A protein 1 family |
Alternative Names
HSSB; MST075; MSTP075; REPA1RF-A protein 1; Replication factor A protein 1; replication protein A 70 kDa DNA-binding subunit; replication protein A1 (70kD); replication protein A1, 70kDa; RF-A; RP-A; RPA70RP-A p70; Single-stranded DNA-binding protein
Molecular Weight
Mass (kDA):
68.138 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 17p13.3 |
| Sequence: | 17; NC_000017.11 (1830005..1900082) |
Subcellular Localizations
Nucleus. Nucleus, PML body. Enriched in PML bodies in cells displaying alternative lengthening of their telomeres.
Diseases
- Neoplasms
- Malignant Neoplasms
- Xeroderma Pigmentosum
- Xeroderma
- Ataxia Telangiectasia
- Xeroderma Pigmentosum Complementation Group A
- Ataxia
- Genomic Instability
- Carcinoma
- Telangiectasis
- Werner Syndrome
- Hereditary Diseases
- Mammary Neoplasms
Interacting Proteins
- BLM
- MTUS2
- POLA1
- PRIMPOL
- RPA2
- RPA3
- WRN
Pathways
- Dna Replication
- Dna Repair
- Cell Cycle
- Single-stranded Dna Binding
- S Phase
- Localization
- Hyperphosphorylation
- Double-strand Break Repair
- Cell Cycle Arrest
- Dna Recombination
- Mismatch Repair
- Cell Death
- Meiosis
PTMs
- Phosphorylation
- Cleavage
- Sumoylation
- Reduction
- Dephosphorylation
- Methylation
Research Areas
- DNA Repair
- Nucleotide Excision Repair
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RPA1
bosterbio.com/bosterbio-gene-info-cards/RPA1
References
PMID: 2050703 by Erdile L.F., et al. Characterization of a cDNA encoding the 70-kDa single-stranded DNA- binding subunit of human replication protein A and the role of the protein in DNA replication.
PMID: 8420996 by Erdile L.F., et al. Type I human complement C2 deficiency. A 28-base pair gene deletion causes skipping of exon 6 during RNA splicing.