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- Table of Contents
RPN1 Antibodies
AND RPN1 ELISA kits, RPN1 Proteins
Many biological assays use antibodies to detect RPN1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RPN1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RPN1 antibodies...
Designing an IHC experiment for RPN1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the RPN1 IHC GuideRPN1 Infographic by Boster Bio
All RPN1 Products
Product
Figures
Specificity
Applications
Price
Human, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M05063-1
100 µl/vial
Cat # M05063-1
100 µl/vial
Human, Mouse, Rat
ELISA
$480
Cat # EKC1790
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1790
1 kit, containing one 96-well plate and all necessary reagents
RPN1 Overview
Facts about Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1)
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the first transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) in the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X- Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).
All subunits are required for a maximum enzyme activity. .
Gene Information
| Human | |
|---|---|
| Gene Name: | RPN1 |
| Uniprot: | P04843 |
| Entrez: | 6184 |
Family
| Belongs to: |
|---|
| OST1 family |
Alternative Names
DKFZp686B16177; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC 2.4.1.119; oligosaccharyltransferase 1 homolog; OST1; ribophorin IRBPH1; ribophorin-1; RPN-I
Molecular Weight
Mass (kDA):
68.569 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3q21.3 |
| Sequence: | 3; NC_000003.12 (128619969..128650818, complement) |
Tissue Specificity
Expressed in all tissues tested.
Subcellular Localizations
Endoplasmic reticulum. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Diseases
- Leukemia
- Leukemia, Myelocytic, Acute
- Myeloid Leukemia
- Malignant Neoplasms
- Dysmyelopoietic Syndromes
- Cytogenetic Abnormality
- Dysplasia
- Leukemogenesis
- Neoplasms
- Chromosome Breakage
- Chromosome Inversion
- Myeloid Leukemia, Chronic
Interacting Proteins
- SGTA
- STT3B
- UBQLN1
Pathways
- Proteolysis
- Dna Repair
- Methylation
- Cell Division
- Proteasome Assembly
- Pathogenesis
- Reverse Transcription
- Induction Of Apoptosis
- Interphase
PTMs
- Methylation
- Phosphorylation
- Glutathionylation
- Cleavage
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RPN1
bosterbio.com/bosterbio-gene-info-cards/RPN1
References
PMID: 3034581 by Crimaudo C., et al. Human ribophorins I and II: the primary structure and membrane topology of two highly conserved rough endoplasmic reticulum-specific glycoproteins.
PMID: 12643545 by Basrur V., et al. Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.