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- Table of Contents
RUNX3 · Western blot design guide
RUNX3 Western Blot Planning Guide
Plan a RUNX3 Western blot around the catalog-observed 44.4 kDa band, image-backed PA2112 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
RUNX3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 44.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Skin | |
| Negative control | Bronchus |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 44.4 kDa |
| ⓘ | Localization | Nucleus / Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Mouse / Rat / Human |
Section 1
Real Curated RUNX3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Skin |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2112 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected RUNX3 Western Blot Band Size?
Use the product-observed 44.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 44.4 kDa | Matches the authoritative product WB observation. |
| 44.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected RUNX3 appearancePlan around 44.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 44.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 44.4 kDa from UniProt Q13761; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2112. |
| Situation | Likely cause | Next action |
|---|---|---|
| 44.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing RUNX3 band
PA2112 · RUNX3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RUNX3 Western blot
🧪Use Skin as the first positive-control candidate and Bronchus as the HPA Not detected negative candidate.
Positive control: Skin (Medium)
Negative control: Bronchus (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for RUNX3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Skin | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Small intestine | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced RUNX3 Western Blot Tips
Deeper troubleshooting and optimisation questions for RUNX3, answered from its protein features.
Which band should guide the blot?
Use 44.4 kDa, the observation attached to the authoritative PA2112 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 44.4 kDa expectation.
Which positive control should I start with?
Start with Skin, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bronchus as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for RUNX3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2112 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
RUNX3 Western Blot Reagents
Mouse/Rat/Human-reactive RUNX3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-RUNX3 Antibody Picoband®
Cat # PA2112
Real WB data
Anti-RUNX3 Antibody Picoband®
Cat # PB9432
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13761
- Human Protein Atlas — RUNX3
- PA2112 product record
- PMC3022884 — Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis (BMC cancer, 2011)
- PMC8027031 — RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis (Cell death and differentiation, 2021)
- PMC5528422 — Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer (Molecular oncology, 2013)