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- Table of Contents
SEC23A · Western blot design guide
SEC23A Western Blot Planning Guide
Plan a SEC23A Western blot around the catalog-observed 86.2 kDa band, image-backed A05287-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SEC23A Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 86.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Stomach | |
| Negative control | Smooth muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 86.2 kDa |
| ⓘ | Localization | Cytoplasmic vesicle, COPII-coated vesicle membrane / Endoplasmic reticulum membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated SEC23A Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebral cortex |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A05287-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SEC23A Western Blot Band Size?
Use the product-observed 86.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 86.2 kDa | Matches the authoritative product WB observation. |
| 86.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SEC23A appearancePlan around 86.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 86.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 86.2 kDa from UniProt Q15436; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A05287-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 86.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SEC23A band
A05287-2 · SEC23A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SEC23A Western blot
🧪Use Stomach as the first positive-control candidate and Smooth muscle as the HPA Not detected negative candidate.
Positive control: Stomach (High)
Negative control: Smooth muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SEC23A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Stomach | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | High | Protein (HPA) | HPA → |
| Nasopharynx | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced SEC23A Western Blot Tips
Deeper troubleshooting and optimisation questions for SEC23A, answered from its protein features.
Which band should guide the blot?
Use 86.2 kDa, the observation attached to the authoritative A05287-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 86.2 kDa expectation.
Which positive control should I start with?
Start with Stomach, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Smooth muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SEC23A Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A05287-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC7435177, PMC7307210, PMC4906273.
Boster reagents
SEC23A Western Blot Reagents
Human/Mouse/Rat-reactive SEC23A Western blot reagents with authoritative product imagery.
Real WB data
Anti-SEC23A Antibody Picoband®
Cat # A05287-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q15436
- Human Protein Atlas — SEC23A
- A05287-2 product record
- PMC7435177 — S100A8 transported by SEC23A inhibits metastatic colonization via autocrine activation of autophagy.
- PMC7307210 — Small sequence variations between two mammalian paralogs of the small GTPase SAR1 underlie functional differences in coat protein complex II assembly.
- PMC4906273 — Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice.