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- Table of Contents
SIRT7 · Western blot design guide
SIRT7 Western Blot Planning Guide
Plan a SIRT7 Western blot around the catalog-observed 44.9 kDa band, image-backed PB9358 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SIRT7 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 44.9 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cerebellum | |
| Negative control | Liver |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 44.9 kDa |
| ⓘ | Localization | Nucleus, nucleolus / Nucleus, nucleoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated SIRT7 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9358 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SIRT7 Western Blot Band Size?
Use the product-observed 44.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 44.9 kDa | Matches the authoritative product WB observation. |
| 44.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SIRT7 appearancePlan around 44.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 44.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 44.9 kDa from UniProt Q9NRC8; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9358. |
| Situation | Likely cause | Next action |
|---|---|---|
| 44.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SIRT7 band
PB9358 · SIRT7
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SIRT7 Western blot
🧪Use Cerebellum as the first positive-control candidate and Liver as the HPA Not detected negative candidate.
Positive control: Cerebellum (Medium)
Negative control: Liver (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SIRT7
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced SIRT7 Western Blot Tips
Deeper troubleshooting and optimisation questions for SIRT7, answered from its protein features.
Which band should guide the blot?
Use 44.9 kDa, the observation attached to the authoritative PB9358 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 44.9 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Liver as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SIRT7 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9358 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
SIRT7 Western Blot Reagents
Human/Mouse/Rat-reactive SIRT7 Western blot reagents with authoritative product imagery.
Real WB data
Anti-SIRT7 Antibody Picoband®
Cat # PB9358
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9NRC8
- Human Protein Atlas — SIRT7
- PB9358 product record
- PMC11295095 — Mechanism of desuccinylation of G6PD mediated by SIRT7 to promote vitiligo disease progression (Immunity, inflammation and disease, 2024)
- PMC4987533 — Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome (Nature communications, 2016)
- PMC12919253 — SIRT7 safeguards ERα proteostasis via deacetylation-dependent degradation of unliganded and misfolded receptors (The Journal of biological chemistry, 2026)
