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- Table of Contents
SLC34A2 · Western blot design guide
SLC34A2 Western Blot Planning Guide
Plan a SLC34A2 Western blot around the catalog-observed 75.8 kDa band, image-backed A03957-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SLC34A2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 75.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Lung | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 75.8 kDa |
| ⓘ | Localization | Apical cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated SLC34A2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bronchus |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03957-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SLC34A2 Western Blot Band Size?
Use the product-observed 75.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 75.8 kDa | Matches the authoritative product WB observation. |
| 75.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SLC34A2 appearancePlan around 75.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 75.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 75.8 kDa from UniProt O95436; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03957-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 75.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SLC34A2 band
A03957-1 · SLC34A2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SLC34A2 Western blot
🧪Use Lung as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Lung (High)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SLC34A2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced SLC34A2 Western Blot Tips
Deeper troubleshooting and optimisation questions for SLC34A2, answered from its protein features.
Which band should guide the blot?
Use 75.8 kDa, the observation attached to the authoritative A03957-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 75.8 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SLC34A2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03957-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
SLC34A2 Western Blot Reagents
Human/Mouse/Rat-reactive SLC34A2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-SLC34A2 Antibody Picoband®
Cat # A03957-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O95436
- Human Protein Atlas — SLC34A2
- A03957-1 product record
- PMC7838622 — Knockdown of SLC34A2 Inhibits Hepatocellular Carcinoma Cell Proliferation and Invasion (Oncology research, 2016)
- PMC11168907 — SLC34A2 promotes cell proliferation by activating STX17-mediated autophagy in esophageal squamous cell carcinoma (Thoracic cancer, 2024)
- PMC5386463 — Overexpression of SLC34A2 is an independent prognostic indicator in bladder cancer and its depletion suppresses tumor growth via decreasing c-Myc expression and transcriptional activity (Cell death & disease, 2017)