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- Table of Contents
TIAM1 · Western blot design guide
TIAM1 Western Blot Planning Guide
Plan a TIAM1 Western blot around the catalog-observed 177.5 kDa band, image-backed PB10103 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
TIAM1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 177.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Cerebellum | |
| Negative control | Adipose tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 177.5 kDa |
| ⓘ | Localization | Cell junction / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated TIAM1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Cerebellum |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB10103 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected TIAM1 Western Blot Band Size?
Use the product-observed 177.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 177.5 kDa | Matches the authoritative product WB observation. |
| 177.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected TIAM1 appearancePlan around 177.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 177.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 177.5 kDa from UniProt Q13009; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB10103. |
| Situation | Likely cause | Next action |
|---|---|---|
| 177.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing TIAM1 band
PB10103 · TIAM1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TIAM1 Western blot
🧪Use Cerebellum as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.
Positive control: Cerebellum (High)
Negative control: Adipose tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for TIAM1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced TIAM1 Western Blot Tips
Deeper troubleshooting and optimisation questions for TIAM1, answered from its protein features.
Which band should guide the blot?
Use 177.5 kDa, the observation attached to the authoritative PB10103 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 177.5 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adipose tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for TIAM1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB10103 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
TIAM1 Western Blot Reagents
Human-reactive TIAM1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-TIAM1 Antibody Picoband®
Cat # PB10103
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13009
- Human Protein Atlas — TIAM1
- PB10103 product record
- PMC2185563 — Ankyrin-Tiam1 interaction promotes Rac1 signaling and metastatic breast tumor cell invasion and migration (The Journal of cell biology, 2000)
- PMC2841637 — Tiam1 as a signaling mediator of nerve growth factor-dependent neurite outgrowth (PloS one, 2010)
