TNFRSF21 · Western blot design guide

TNFRSF21 Western Blot Planning Guide

Plan a TNFRSF21 Western blot around the catalog-observed 71.8 kDa band, image-backed A04348-2 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for TNFRSF21 (TNFRSF21): expected band 71.8 kDa, antibody A04348-2, and PMC-cited SDS-PAGE protocol steps — TNFRSF21 Western blot protocol sheet — expected band 71.8 kDa, antibody A04348-2, controls and PMC citations. Open the full TNFRSF21 WB guide →

Navigate to: Protocols Band Controls Antibodies

TNFRSF21 Western Blot Experimental Design Guide

Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	71.8 kDa
	Observed band	Not reported — verify product WB image
	Gel	12-15%
	Positive control ⓘ	Cerebellum
	Negative control ⓘ	Bone marrow

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	71.8 kDa
ⓘ	Localization	Cell membrane
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Section 1

Real Curated TNFRSF21 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Cerebellum
Gel %	12-15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A04348-2 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected TNFRSF21 Western Blot Band Size?

Use the product-observed 71.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
71.8 kDa	Matches the authoritative product WB observation.
71.8 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected TNFRSF21 appearancePlan around 71.8 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	71.8 kDa; use this as the primary experimental expectation.
Calculated mass	71.8 kDa from UniProt O75509; retain as context.
Gel selection	12-15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A04348-2.

Why is my band missing or off?
Situation	Likely cause	Next action
71.8 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt O75509 Human Protein Atlas product datasheet (A04348-2)

Experiment evidence

Western blot images showing TNFRSF21 band

A04348-2 · TNFRSF21

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for TNFRSF21 Western blot

🧪Use Cerebellum as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.

Positive control: Cerebellum (High)

Negative control: Bone marrow (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for TNFRSF21

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cerebellum	Reported tissue cells	High	Protein (HPA)	HPA →
Hippocampus	Reported tissue cells	High	Protein (HPA)	HPA →
Cerebral cortex	Reported tissue cells	High	Protein (HPA)	HPA →
Adipose tissue	Reported tissue cells	Medium	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Bone marrow	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced TNFRSF21 Western Blot Tips

Deeper troubleshooting and optimisation questions for TNFRSF21, answered from its protein features.

Which band should guide the blot?

Use 71.8 kDa, the observation attached to the authoritative A04348-2 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 71.8 kDa expectation.

Which positive control should I start with?

Start with Cerebellum, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Bone marrow as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 12-15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for TNFRSF21 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A04348-2 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC9220259, PMC7202486, PMC6487688.

Boster reagents

TNFRSF21 Western Blot Reagents

Human/Mouse/Rat-reactive TNFRSF21 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for TNFRSF21 using A04348-2; observed band 71.8 kDa

Anti-DR6/TNFRSF21 Antibody Picoband®

Cat # A04348-2

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.