E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)

Functions as a ubiquitin E3 ligase as Well as an ISG15 E3 ligase.

Involved in innate immune defense against viruses by mediating ubiquitination of DDX58.

Mediates'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like area that's crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway.