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- Table of Contents
TRPM7 · Western blot design guide
TRPM7 Western Blot Planning Guide
Plan a TRPM7 Western blot around the catalog-observed 212.7 kDa band, image-backed A00789-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
TRPM7 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 212.7 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Parathyroid gland | |
| Negative control | Cerebral cortex |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 212.7 kDa |
| ⓘ | Localization | Cell membrane / Cytoplasmic vesicle membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated TRPM7 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Parathyroid gland |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00789-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected TRPM7 Western Blot Band Size?
Use the product-observed 212.7 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 212.7 kDa | Matches the authoritative product WB observation. |
| 212.7 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected TRPM7 appearancePlan around 212.7 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 212.7 kDa; use this as the primary experimental expectation. |
| Calculated mass | 212.7 kDa from UniProt Q96QT4; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00789-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 212.7 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing TRPM7 band
A00789-1 · TRPM7
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TRPM7 Western blot
🧪Use Parathyroid gland as the first positive-control candidate and Cerebral cortex as the HPA Not detected negative candidate.
Positive control: Parathyroid gland (High)
Negative control: Cerebral cortex (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for TRPM7
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Parathyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced TRPM7 Western Blot Tips
Deeper troubleshooting and optimisation questions for TRPM7, answered from its protein features.
Which band should guide the blot?
Use 212.7 kDa, the observation attached to the authoritative A00789-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 212.7 kDa expectation.
Which positive control should I start with?
Start with Parathyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Cerebral cortex as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for TRPM7 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00789-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
TRPM7 Western Blot Reagents
Human/Mouse/Rat-reactive TRPM7 Western blot reagents with authoritative product imagery.
Real WB data
Anti-TRPM7 Antibody Picoband®
Cat # A00789-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q96QT4
- Human Protein Atlas — TRPM7
- A00789-1 product record
- PMC7768084 — TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma (Frontiers in pharmacology, 2020)
- PMC3442166 — Evidence for functional expression of TRPM7 channels in human atrial myocytes (Basic research in cardiology, 2012)
- PMC9271301 — TRPM7 via calcineurin/NFAT pathway mediates metastasis and chemotherapeutic resistance in head and neck squamous cell carcinoma (Aging, 2022)
