Tubulin beta-2A chain Antibodies

AND TUBB2A ELISA kits, Tubulin beta-2A chain Proteins

Many biological assays use antibodies to detect Tubulin beta-2A chain, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Tubulin beta-2A chain in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Tubulin beta-2A chain antibodies...

We validate the specificity of these antibodies to Tubulin beta-2A chain by testing them on tissues known to express TUBB2A positively and negatively. Browse below to find the TUBB2A antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Tubulin beta-2A chain? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the TUBB2A Western Blot Guide

TUBB2A Infographic by Boster Bio

All TUBB2A Products

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Applications

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Anti-beta II Tubulin Antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0

All lanes : Anti-beta II Tubulin at 1:8000 dilutionLane 1: mouse brain lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: PC-12 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane

Overlay histogram showing NIH/3T3 cells stained with M06868-1 (green line)

Human, Mouse, Rat

Flow Cytometry, IF, WB

$299
Cat # M06868-1
50 µl

Anti-beta II tubulin TUBB2A Monoclonal Antibody

Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, diluted at 1:100000

1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1: 200 Western blot analysis: primary antibody : TDY191 1: 10000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1: 5000

Immunohistochemical analysis of paraffin-embedded Human-colon tissue

Human, Mouse, Rat

IP, IF, IHC, WB

$380
Cat # M06868
100ul

beta II Tubulin (TUBB2A) (NM_001069) Human Over-expression Lysate

Human

$628
Cat # LS007280
20 µg, 100 µg

TUBB2A Overview

Facts about Tubulin beta-2A chain.

TUBB2A 3D structure. Source: alphafold

Tubulin beta-2A chain (TUBB2A)

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

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Gene Information

Human
Gene Name:	TUBB2A
Uniprot:	Q13885
Entrez:	7280

Family

Belongs to:

tubulin family

Alternative Names

dJ40E16.7; TUBB; TUBB2; TUBB2B; tubulin beta-2A chain; tubulin, beta 2; tubulin, beta 2A; tubulin, beta polypeptide 2; tubulin, beta polypeptide

Molecular Weight

Mass (kDA):
49.907 kDA

Genomic Context


Human

Location:	6p25.2
Sequence:	6; NC_000006.12 (3153666..3157544, complement)

Tissue Specificity

High expression in brain, where it represents 30% of all beta-tubulins.

Subcellular Localizations

Cytoplasm, cytoskeleton.

Diseases

Neoplasms
Malignant Neoplasms
Adenocarcinoma
Malignant Neoplasm Of Prostate
Neoplasm Metastasis
Lung Neoplasms
Non-small Cell Lung Carcinoma
Primary Neoplasm
Malignant Neoplasm Of Breast
Neurotoxicity Syndromes
Malignant Neoplasm Of Lung

Neoplasms, Hormone-dependent
Prostatic Neoplasms
Peripheral Neuropathy
Mammary Neoplasms
Microtubule Process
Dysequilibrium Syndrome

Interacting Proteins

ACD
LRRK2

Pathways

Cell Division
Drug Resistance
Translation
Cell Cycle
Response To Paclitaxel
Cell Cycle Arrest

PTMs

Acetylation
Isopeptide bond

Methylation
Phosphoprotein

Ubl conjugation

Research Areas

Apoptosis
Cell Biology
Cellular Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TUBB2A

References

PMID: 16371510 by Fourest-Lieuvin A., et al. Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.

PMID: 19131338 by Garapaty S., et al. Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation.