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- Table of Contents
TRIM25 Antibodies
AND TRIM25 ELISA kits, TRIM25 Proteins
Many biological assays use antibodies to detect TRIM25, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with TRIM25 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop TRIM25 antibodies...
Designing a Western blot for TRIM25? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the TRIM25 Western Blot GuideDesigning an IHC experiment for TRIM25? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the TRIM25 IHC GuideTRIM25 Infographic by Boster Bio
All TRIM25 Products
Product
Figures
Specificity
Applications
Price
Human
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A03232-1
100 μg/vial
Cat # A03232-1
100 μg/vial
Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A03232-4
100 μg/vial
Cat # A03232-4
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # M03232
100 μl
Cat # M03232
100 μl
Human
ELISA
$480
Cat # EKC1807
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1807
1 kit, containing one 96-well plate and all necessary reagents
TRIM25 Overview
Facts about E3 ubiquitin/ISG15 ligase TRIM25.
E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)
Functions as a ubiquitin E3 ligase as Well as an ISG15 E3 ligase.
Involved in innate immune defense against viruses by mediating ubiquitination of DDX58.Mediates'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like area that's crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway.
Gene Information
| Human | |
|---|---|
| Gene Name: | TRIM25 |
| Uniprot: | Q14258 |
| Entrez: | 7706 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
EC 6.3.2.19; EC 6.3.2.n3; EFPZ147; Estrogen-responsive finger protein; RING finger protein 147; RNF147E3 ubiquitin/ISG15 ligase TRIM25; tripartite motif containing 25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein); Zinc finger protein 147; zinc finger protein-147; ZNF147tripartite motif protein TRIM25
Molecular Weight
Mass (kDA):
70.973 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 17q22 |
| Sequence: | 17; NC_000017.11 (56887909..56914049, complement) |
Tissue Specificity
Expressed in breast tumors (at protein level). Ubiquitous.
Subcellular Localizations
Cytoplasm. Cytoplasm, Stress granule.
Diseases
- Malignant Neoplasms
- Virus Diseases
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Neoplasms
- Influenza
- Innate Immune Response
- Carcinoma
- Adenocarcinoma
- Carcinogenesis
- Rna Virus Infections
- Endometrial Polyp
- Endometrial Adenocarcinoma
Interacting Proteins
- DDX58
Pathways
- Cell Proliferation
- Immune Response
- Cell Growth
- Proteolysis
- Type I Interferon Production
- Translation
- Innate Immune Response
- Cell Cycle
- Conjugation
- Localization
- Viral Replication
- Rig-i Signaling Pathway
- Virulence
PTMs
- Ubiquitination
- Phosphorylation
- Isgylation
Research Areas
- Cancer
- Zinc Finger
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRIM25
bosterbio.com/bosterbio-gene-info-cards/TRIM25
References
PMID: 8248217 by Inoue S., et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
PMID: 15130519 by Shimada N., et al. Systemic distribution of estrogen-responsive finger protein (Efp) in human tissues.