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- Table of Contents
UNG · Western blot design guide
UNG Western Blot Planning Guide
Plan an UNG Western blot around the catalog-observed 34.6 kDa band, image-backed A01672-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
UNG Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 34.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Adrenal gland | |
| Negative control | Smooth muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 34.6 kDa |
| ⓘ | Localization | Mitochondrion / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated UNG Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01672-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected UNG Western Blot Band Size?
Use the product-observed 34.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 34.6 kDa | Matches the authoritative product WB observation. |
| 34.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected UNG appearancePlan around 34.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 34.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 34.6 kDa from UniProt P13051; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01672-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 34.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing UNG band
A01672-2 · UNG
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UNG Western blot
🧪Use Adrenal gland as the first positive-control candidate and Smooth muscle as the HPA Not detected negative candidate.
Positive control: Adrenal gland (High)
Negative control: Smooth muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for UNG
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced UNG Western Blot Tips
Deeper troubleshooting and optimisation questions for UNG, answered from its protein features.
Which band should guide the blot?
Use 34.6 kDa, the observation attached to the authoritative A01672-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 34.6 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Smooth muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for UNG Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01672-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC4201938, PMC3944228, PMC9425297.
Boster reagents
UNG Western Blot Reagents
Human/Mouse/Rat-reactive UNG Western blot reagents with authoritative product imagery.
Real WB data
Anti-UNG Antibody Picoband®
Cat # A01672-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P13051
- Human Protein Atlas — UNG
- A01672-2 product record
- PMC4201938 — Uracil-DNA glycosylase expression determines human lung cancer cell sensitivity to pemetrexed.
- PMC3944228 — Uracil DNA glycosylase (UNG) loss enhances DNA double strand break formation in human cancer cells exposed to pemetrexed.
- PMC9425297 — FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation.