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Product Info Summary
| SKU: | A06193-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CAT2/SLC7A2 Antibody Picoband®
SKU/Catalog Number
A06193-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CAT2/SLC7A2 Antibody Picoband® catalog # A06193-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CAT2/SLC7A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06193-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CAT2/SLC7A2 recombinant protein (Position: M1-F658).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06193-1 is reactive to SLC7A2 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
71.7 kDa
Background of SLC7A2
Cationic amino acid transporter 2 is a protein that in humans is encoded by the SLC7A2 gene. The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06193-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human placenta tissue, rat skeletal muscle tissue, rat ovary tissue, mouse skeletal muscle tissue, mouse C2C12 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of CAT2/SLC7A2 using anti-CAT2/SLC7A2 antibody (A06193-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat ovary tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAT2/SLC7A2 antigen affinity purified polyclonal antibody (Catalog # A06193-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAT2/SLC7A2 at approximately 100 kDa. The expected band size for CAT2/SLC7A2 is at 72 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-CAT2/SLC7A2 antibody (A06193-1).
Overlay histogram showing HepG2 cells stained with A06193-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CAT2/SLC7A2 Antibody (A06193-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-CAT2/SLC7A2 Antibody Picoband® (A06193-1)
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